ArticleGenetics and molecular biology2017
Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean.
Article in Genetics and molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 37 citations in OpenAlex.
- Characterization, evolution, and abiotic stress responses of leucine-rich repeat receptor-like protein kinases (LRR-RLK) in Liriodendron chinense.BMC genomics · 2024Article
- Structural and functional analysis of stress-inducible genes and their promoters selected from young oil palm (Elaeis guineensis) under salt stress.BMC genomics · 2022Article
- Isolation and characterization of drought and ABA responsive promoter of a transcription factor encoding gene from rice.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Discovery and functional characterization of novel cotton promoters with potential application to pest control.Plant cell reports · 2022Article
- Functional and molecular characterization of fluoride exporter (FEX) from rice and its constitutive overexpression in Nicotiana benthamiana to promote fluoride tolerance.Plant cell reports · 2021Article
- An update and perspectives on the use of promoters in plant genetic engineering.Journal of biosciences · 2020Review
- Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application.BMC biotechnology · 2019Article
- Development and validation of a novel and robust cell culture system in soybean (Glycine max (L.) Merr.) for promoter screening.Plant cell reports · 2019Article
- Article
- Molecular characterization and differential expression suggested diverse functions of P-type II CaBMC genomics · 2018Article
- Genome-wide identification of the auxin response factor gene family in Cicer arietinum.BMC genomics · 2018Article
- Genome-scale examination of NBS-encoding genes in blueberry.Scientific reports · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drought stress is the main limiting factor of soybean yield. Currently, genetic engineering has been one important tool in the development of drought-tolerant cultivars. A widely used strategy is the fusion of genes that confer tolerance under the control of the CaMV35S constitutive promoter; however, stress-responsive promoters would constitute the best alternative to the generation of drought-tolerant crops. We characterized the promoter of α-galactosidase soybean (GlymaGAL) gene that was previously identified as highly up-regulated by drought stress. The β-glucuronidase (GUS) activity of Arabidopsis transgenic plants bearing 1000- and 2000-bp fragments of the GlymaGAL promoter fused to the uidA gene was evaluated under air-dried, polyethylene glycol (PEG) and salt stress treatments. After 24 h of air-dried and PEG treatments, the pGAL-2kb led to an increase in GUS expression in leaf and root samples when compared to the control samples. These results were corroborated by qPCR expression analysis of the uidA gene. The pGAL-1kb showed no difference in GUS activity between control and treated samples. The pGAL-2kb promoter was evaluated in transgenic soybean roots, leading to an increase in EGFP expression under air-dried treatment. Our data indicates that pGAL-2kb could be a useful tool in developing drought-tolerant cultivars by driving gene expression.
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