ArticleScientific reports2017
Potential of MALDI-TOF MS as an alternative approach for capsular typing Streptococcus pneumoniae isolates.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Antibiotic Resistance and Biofilm-Forming Capacity of Oral Biofilm Bacteria in Patients With Periodontitis and Healthy Individuals.MicrobiologyOpen · 2026Article
- Matrix-assisted laser desorption/ionization-time of flight MS as a tool for clonal discrimination inJournal of medical microbiology · 2025Article
- Analysis of high-molecular-weight proteins using MALDI-TOF MS and machine learning for the differentiation of clinically relevant Clostridioides difficile ribotypes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Polyphasic identification of Vibrio species from aquatic sources using mass spectrometry, housekeeping gene sequencing and whole genome analysis.Scientific reports · 2024Article
- MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory.Microorganisms · 2024Review
- ExploringJournal of mass spectrometry and advances in the clinical lab · 2023Article
- MALDI-TOF MS: An alternative approach for ribotyping Clostridioides difficile isolates in Brazil.Anaerobe · 2021Article
- MALDI-TOF Mass Spectroscopy Applications in Clinical Microbiology.Advances in pharmacological and pharmaceutical sciences · 2021Review
- MALDI-TOF mass spectrometry for sub-typing of Streptococcus pneumoniae.BMC microbiology · 2020Article
- Characterization ofMicroorganisms · 2020Article
- MALDI-TOF Mass Spectrometry and Specific Biomarkers: Potential New Key for Swift Identification of Antimicrobial Resistance in Foodborne Pathogens.Microorganisms · 2019Review
- Pathogen proteotyping: A rapidly developing application of mass spectrometry to address clinical concerns.Clinical mass spectrometry (Del Mar, Calif.) · 2019Review
- Article
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8 authors.
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Abstract
Streptococcus pneumoniae can be classified in more than 90 capsular types, as traditionally determined by serological methods and more recently by PCR-based techniques. Such methods, however, can be expensive, laborious or unable to accurately discriminate among certain serotypes. Therefore, determination of capsular types, although extremely important for epidemiological purposes and for estimating the impact of pneumococcal conjugate vaccines, is mainly restricted to research laboratories, being rarely performed in the clinical setting. In the present study, MALDI-TOF MS was evaluated as an alternative tool to characterize 416 pneumococcal isolates belonging to serotypes 6A, 6B, 6C, 9N, 9V or 14. For MALDI-TOF MS analysis, each isolate was submitted to an extraction protocol using formic acid and acetonitrile. Measurements were performed with a Bruker Microflex LT mass spectrometer using default parameters and generating spectra in the range of 2,000-20,000 m/z. Spectra were analyzed with the BioNumerics software v7.6. Isolates were mainly distributed according to the capsular type in a Neighbor Joining tree and serotypes investigated were successfully discriminated by the presence/absence of 14 selected biomarkers. The results suggest that MALDI-TOF MS is a promising alternative for typing pneumococcal strains, highlighting its usefulness for rapid and cost-effective routine application in clinical laboratories.
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