Evidence map›Paper›PMID 28349967›Full record

ArticleScientific reports2017

Development of a multiplex loop-mediated isothermal amplification method for the simultaneous detection of Salmonella spp. and Vibrio parahaemolyticus.

Ningwei Liu, Dayang Zou, Derong Dong, Zhan Yang, Da Ao, Wei Liu, Liuyu Huang

Open access · goldAbstract readEvaluation Study
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
7.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 84 citations in OpenAlex.

  1. Article
  2. Review
  3. Public health aspects ofEFSA journal. European Food Safety Authority · 2024
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  6. Recent Developments in Lateral Flow Assays forPathogens (Basel, Switzerland) · 2023
    Review
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Rapid and Visual Detection ofJournal of microbiology and biotechnology · 2021
    Article
  15. Advancement inBiosensors · 2021
    Review
  16. Article
  17. Article
  18. DNA Transformations for Diagnosis and Therapy.Advanced functional materials · 2021
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Ningwei LiuInstitute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Dayang ZouInstitute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Derong DongInstitute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Zhan YangInstitute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Da AoInstitute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Wei LiuInstitute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Liuyu HuangInstitute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Academy of Military Medical Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapid detection of food-borne pathogens is important in the food industry, to monitor and prevent the spread of these pathogens through contaminated food products. We therefore established a multiplex real-time loop-mediated isothermal amplification (LAMP) assay to simultaneously detect and distinguish Salmonella spp. and Vibrio parahaemolyticus DNA in a single reaction. Two target sequences, one specific for Salmonella and the other specific for Vibrio parahaemolyticus, were amplified by specific LAMP primers in the same reaction tube. After amplification at 65 °C for 60 min, the amplified products were subjected to melting curve analysis and thus could be distinguished based on the different melting temperatures (T

Indexed as

Food MicrobiologyMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesSalmonellaSensitivity and SpecificityTemperatureTimeTransition TemperatureVibrio parahaemolyticus

Identifiers

PMID28349967
PMCPMC5368564
OpenAlexW2600607573

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.