ArticleMicrobiologyOpen2017
Metabolic engineering to expand the substrate spectrum of Pseudomonas putida toward sucrose.
Article in MicrobiologyOpen, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Enhanced sucrose production by controlling carbon flux through CfrA expression in Synechocystis sp. PCC 6803.Microbial cell factories · 2025Article
- Cellulase secretion by engineered Pseudomonas putida enables growth on cellulose oligomers.Applied microbiology and biotechnology · 2025Article
- Engineered Sucrose Metabolism Improves the Smut Disease Suppression Potency of Pseudomonas sp. ST4.Applied and environmental microbiology · 2023Article
- Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery.Microbial biotechnology · 2023Review
- Streamlining of a synthetic co-culture towards an individually controllable one-pot process for polyhydroxyalkanoate production from light and COEngineering in life sciences · 2023Article
- D-Xylose Sensing inInternational journal of molecular sciences · 2021Review
- Metabolic engineering of Pseudomonas putida for production of the natural sweetener 5-ketofructose from fructose or sucrose by periplasmic oxidation with a heterologous fructose dehydrogenase.Microbial biotechnology · 2021Article
- Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by ArcticPolymers · 2021Article
- Developing Bioprospecting Strategies for Bioplastics Through the Large-Scale Mining of Microbial Genomes.Frontiers in microbiology · 2021Article
- Industrial biotechnology of Pseudomonas putida: advances and prospects.Applied microbiology and biotechnology · 2020Review
- Biotransformation of 2,4-dinitrotoluene in a phototrophic co-culture of engineered Synechococcus elongatus and Pseudomonas putida.Microbial biotechnology · 2020Article
- Engineering sucrose metabolism in Pseudomonas putida highlights the importance of porins.Microbial biotechnology · 2020Article
- An automated and parallelised DIY-dosing unit for individual and complex feeding profiles: Construction, validation and applications.PloS one · 2019Article
- Metabolic engineering to expand the substrate spectrum of Pseudomonas putida toward sucrose.MicrobiologyOpen · 2017Article
- Photoautotrophic production of polyhydroxyalkanoates in a synthetic mixed culture ofBiotechnology for biofuels · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sucrose is an important disaccharide used as a substrate in many industrial applications. It is a major component of molasses, a cheap by-product of the sugar industry. Unfortunately, not all industrially relevant organisms, among them Pseudomonas putida, are capable of metabolizing sucrose. We chose a metabolic engineering approach to circumvent this blockage and equip P. putida with the activities necessary to consume sucrose. Therefore, we constructed a pair of broad-host range mini-transposons (pSST - sucrose splitting transposon), carrying either cscA, encoding an invertase able to split sucrose into glucose and fructose, or additionally cscB, encoding a sucrose permease. Introduction of cscA was sufficient to convey sucrose consumption and the additional presence of cscB had no further effect, though the sucrose permease was built and localized to the membrane. Sucrose was split extracellularly by the activity of the invertase CscA leaking out of the cell. The transposons were also used to confer sucrose consumption to Cupriavidus necator. Interestingly, in this strain, CscB acted as a glucose transporter, such that C. necator also gained the ability to grow on glucose. Thus, the pSST transposons are functional tools to extend the substrate spectrum of Gram-negative bacterial strains toward sucrose.
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Registered trials
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