ArticleClinical oral investigations2018
Bacterial colonization in the apical part of extracted human teeth following root-end resection and filling: a confocal laser scanning microscopy study.
Article in Clinical oral investigations, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- CLSM-guided imaging provides new insights into position-dependent limitations of endodontic disinfection.Clinical oral investigations · 2026Article
- Assessment of sealing ability, antibacterial effect and marginal adaptation of bioactive materials in pulpotomized primary molars: an in-vitro study.BMC oral health · 2026Article
- Bacterial sealing ability of calcium silicate-based sealer for endodontic surgery: an in-vitro study.BMC oral health · 2024Article
- CLSM-Guided Imaging for Quantifying Endodontic Disinfection.Antibiotics (Basel, Switzerland) · 2024Article
- Minimally invasive management of vital teeth requiring root canal therapy.Scientific reports · 2023Article
- Reparative dentin formation as a possible factor influencing the penetrability of dentin in human teeth with apical periodontitis: an ex vivo study.BMC oral health · 2023Article
- Efficacy of Endodontic Disinfection Protocols in anJournal of functional biomaterials · 2023Article
- Bacterial Colonization and Proliferation in Primary Molars following the Use of the Hall Technique: A Confocal Laser Scanning Microscopy Study.Children (Basel, Switzerland) · 2023Article
- Quantification of Bacterial DNA from Infected Human Root Canals Using qPCR and DAPI after Disinfection with Established and Novel Irrigation Protocols.Materials (Basel, Switzerland) · 2022Article
- Micro-computed tomographic evaluation of the flow and filling ability of endodontic materials using different test models.Restorative dentistry & endodontics · 2020Article
- Quantification of Bacterial Colonization in Dental Hard Tissues Using Optimized Molecular Biological Methods.Frontiers in genetics · 2020Article
- Antimicrobial Activity of Calcium-Enriched Mixture Cement and Biodentine onIranian endodontic journal · 2019Article
- Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen.TheScientificWorldJournal · 2019Article
- How does the pulpal response to Biodentine and ProRoot mineral trioxide aggregate compare in the laboratory and clinic?British dental journal · 2018Article
- Efficacy of Mineral Trioxide Aggregate and Biodentine as Apical Barriers in Immature Permanent Teeth: A Microbiological Study.International journal of clinical pediatric dentistryArticle
- Reformulated mineral trioxide aggregate components and the assessments for use as future dental regenerative cements.Journal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe purpose of this study was to evaluate Enterococcus faecalis colonization at the apical part of root canals following root-end resection and filling using confocal laser scanning microscopy (CLSM). MATERIALS AND
methodsThe apical 3-mm root-ends of 55 extracted single rooted human teeth were resected, and 3-mm retrograde cavities were prepared and filled using either mineral trioxide aggregate (MTA), intermediate restorative material (IRM), or Biodentine (n = 10 each); 25 teeth served as controls. The roots were placed in an experimental model, sterilized, and coronally filled with E. faecalis bacterial suspension for 21 days. Then, the apical 3-mm segments were cut to get two slabs (coronal and apical). The slabs were stained using LIVE/DEAD BacLight Bacterial Viability Kit and evaluated using CLSM.
resultsThe fluorescence-stained areas were larger in the bucco-lingual directions compared with the mesio-distal directions (p < 0.05). The mean and maximal depths of bacterial colonization into the dentinal tubules were 755 and 1643 μm, respectively, with no differences between the root-end filling materials (p > 0.05). However, more live bacteria were found in the MTA group in comparison to IRM and Biodentine groups (p < 0.05).
conclusionsCLSM can be used to histologically demonstrate bacterial root-end colonization following root-end filling. This colonization at the filling-dentine interfaces and deeper into the dentinal tubules may be inhomogeneous, favoring the bucco-lingual aspects of the root. CLINICAL RELEVANCE: Following root-end resection and filling bacterial colonization may lead to inflammatory reactions at the periapical tissues; the viability of the colonized bacteria may be affected by the type of root-end filling material.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.