ArticleScientific reports2017
X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Sparse-Scan Ptychography with Hybrid-Overlap Scanning and Reference-Guided Probe Updates.Sensors (Basel, Switzerland) · 2026Article
- X-ray fluorescence microscopy exposure estimates using a single excitation energy.Journal of synchrotron radiation · 2026Article
- The hard X-ray ptychography endstation at the Taiwan Photon Source.Journal of synchrotron radiation · 2026Article
- Multifunctional bending magnet beamline with a capillary optic for X-ray fluorescence studies of metals in tissue sections.Journal of synchrotron radiation · 2026Article
- Substrate and standard evaluation for correlative elemental mapping of biological samples by X-ray fluorescence microscopy and laser ablation ICP-MS.Journal of analytical atomic spectrometry · 2026Article
- Fourier ptychography microscopy for digital pathology.Journal of microscopy · 2025Review
- Biotransformation and biological fate of magnetic iron oxide nanoparticles for biomedical research and clinical applications.Nanoscale advances · 2025Review
- Enhanced Imaging in Scanning Transmission X-Ray Microscopy Assisted by Ptychography.Nanomaterials (Basel, Switzerland) · 2025Article
- Ptychography at all wavelengths.Nature reviews. Methods primers · 2025Article
- X-ray phase-contrast tomography of cells manipulated with an optical stretcher.Journal of synchrotron radiation · 2024Article
- A small-angle X-ray scattering study of red blood cells in continuous flow.Journal of synchrotron radiation · 2023Article
- Synchrotron-Based Nano-X-Ray Absorption Near-Edge Structure Revealing Intracellular Heterogeneity of Iron Species in Magnetotactic Bacteria.Small science · 2022Article
- Phagosomal chloride dynamics in the alveolar macrophage.iScience · 2022Article
- Dual energy X-ray beam ptycho-fluorescence imaging.Journal of synchrotron radiation · 2021Article
- Off-axis multilayer zone plate with 16 nm × 28 nm focus for high-resolution X-ray beam induced current imaging.Journal of synchrotron radiation · 2021Article
- Single-cell visualization and quantification of trace metals inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Upscaling X-ray nanoimaging to macroscopic specimens.Journal of applied crystallography · 2021Review
- Simultaneous structural and elemental nano-imaging of human brain tissue.Chemical science · 2020Article
- Nano-imaging trace elements at organelle levels in substantia nigra overexpressing α-synuclein to model Parkinson's disease.Communications biology · 2020Article
- X-ray ptychographic mode of self-assembled CdSe/CdS octapod-shaped nanocrystals in thick polymers.Journal of applied crystallography · 2020Article
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Authors and funding
8 authors.
Funding
Abstract
X-ray microscopy can be used to image whole, unsectioned cells in their native hydrated state. It complements the higher resolution of electron microscopy for submicrometer thick specimens, and the molecule-specific imaging capabilites of fluorescence light microscopy. We describe here the first use of fast, continuous x-ray scanning of frozen hydrated cells for simultaneous sub-20 nm resolution ptychographic transmission imaging with high contrast, and sub-100 nm resolution deconvolved x-ray fluorescence imaging of diffusible and bound ions at native concentrations, without the need to add specific labels. By working with cells that have been rapidly frozen without the use of chemical fixatives, and imaging them under cryogenic conditions, we are able to obtain images with well preserved structural and chemical composition, and sufficient stability against radiation damage to allow for multiple images to be obtained with no observable change.
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