ArticleProceedings of the National Academy of Sciences of the United States of America2017
Optical visualization and quantification of enzyme activity using dynamic droplet lenses.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Semiartificial COChemical reviews · 2026Review
- Self-Assembled Nanostructured Microgels with Reconfigurable Morphologies.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- A Machine Vision Perspective on Droplet-Based Microfluidics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Monitoring Enzyme Activity Using Near-Infrared Fluorescent Single-Walled Carbon Nanotubes.ACS sensors · 2024Review
- In situ Tracking of Exoenzyme Activity Using Droplet Luminescence Concentrators for Ratiometric Detection of Bacteria.ACS sensors · 2023Article
- Innovative Real-Time Flow Sensor Using Detergent-Free Complex Emulsions with Dual-Emissive Semi-Perfluoroalkyl Substituted Α-Cyanostilbene.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Responsive Janus droplets as modular sensory layers for the optical detection of bacteria.Analytical and bioanalytical chemistry · 2023Review
- Janus Droplet Formation via Thermally Induced Phase Separation: A Numerical Model with Diffusion and Convection.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Host-guest liquid gating mechanism with specific recognition interface behavior for universal quantitative chemical detection.Nature communications · 2022Article
- Janus Emulsion Biosensors for Anti-SARS-CoV-2 Spike Antibody.ACS central science · 2021Article
- Dynamic Complex Emulsions as Amplifiers for On-Chip Photonic Cavity-Enhanced Resonators.ACS sensors · 2020Article
- Fluorescent Janus emulsions for biosensing ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Rapid Detection ofACS central science · 2019Article
- Waveguide-based chemo- and biosensors: complex emulsions for the detection of caffeine and proteins.Lab on a chip · 2019Article
- Morphology-Dependent Luminescence in Complex Liquid Colloids.Journal of the American Chemical Society · 2019Article
- Emulsion Agglutination Assay for the Detection of Protein-Protein Interactions: An Optical Sensor for Zika Virus.ACS sensors · 2019Article
- Interfacial bioconjugation on emulsion droplet for biosensors.Bioorganic & medicinal chemistry · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrate how simple photodetector measurements may be used to monitor enzyme kinetics. This approach is validated by quantitative measurements of enzyme activity for three different classes of enzymes (amylase, lipase, and sulfatase), relying on two distinct mechanisms for coupling droplet morphology to enzyme activity (host-guest interactions with uncaging and molecular cleavage).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.