ArticleScientific reports2017
Environmental DNA in subterranean biology: range extension and taxonomic implications for Proteus.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 107 citations in OpenAlex.
- Towards evidence-based conservation of subterranean ecosystems.Biological reviews of the Cambridge Philosophical Society · 2022Pooled it
- Article
- Unlocking Demography: Developing an eDNA-Based Toolkit to Measure Sex Ratios From Populations.Molecular ecology resources · 2026Article
- Investigating the impact of the construction of the Duliu River dam in China on the spatiotemporal changes of fish communities.Scientific reports · 2025Article
- Nuclear environmental DNA resolves fine-scale population genetic structure in an aquatic habitat.iScience · 2024Article
- Integrating citizen science and environmental DNA metabarcoding to study biodiversity of groundwater amphipods in Switzerland.Scientific reports · 2023Article
- A validated protocol for eDNA-based monitoring of within-species genetic diversity in a pond-breeding amphibian.Scientific reports · 2023Article
- Assessing the breeding phenology of a threatened frog species using eDNA and automatic acoustic monitoring.PeerJ · 2023Article
- Article
- Development and validation of an environmental DNA assay to detect federally threatened groundwater salamanders in central Texas.PloS one · 2023Article
- A theoretical analysis of taxonomic binning accuracy.Molecular ecology resources · 2022Article
- Living in darkness: Exploring adaptation of Proteus anguinus in 3 dimensions by X-ray imaging.GigaScience · 2022Article
- From Cave Dragons to Genomics: Advancements in the Study of Subterranean Tetrapods.Bioscience · 2022Article
- Cultivable Skin Mycobiota of Healthy and Diseased Blind Cave Salamander (Frontiers in microbiology · 2022Article
- Inferring predator-prey interaction in the subterranean environment: a case study from Dinaric caves.Scientific reports · 2021Article
- Collecting eco-evolutionary data in the dark: Impediments to subterranean research and how to overcome them.Ecology and evolution · 2021Review
- Leveraging eDNA to expand the study of hybrid zones.Molecular ecology · 2020Article
- Population-level inferences from environmental DNA-Current status and future perspectives.Evolutionary applications · 2020Review
- Article
- Review
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 5 countries.
Funding
Abstract
Europe's obligate cave-dwelling amphibian Proteus anguinus inhabits subterranean waters of the north-western Balkan Peninsula. Because only fragments of its habitat are accessible to humans, this endangered salamander's exact distribution has been difficult to establish. Here we introduce a quantitative real time polymerase chain reaction-based environmental DNA (eDNA) approach to detect the presence of Proteus using water samples collected from karst springs, wells or caves. In a survey conducted along the southern limit of its known range, we established a likely presence of Proteus at seven new sites, extending its range to Montenegro. Next, using specific molecular probes to discriminate the rare black morph of Proteus from the closely related white morph, we detected its eDNA at five new sites, thus more than doubling the known number of sites. In one of these we found both black and white Proteus eDNA together. This finding suggests that the two morphs may live in contact with each other in the same body of groundwater and that they may be reproductively isolated species. Our results show that the eDNA approach is suitable and efficient in addressing questions in biogeography, evolution, taxonomy and conservation of the cryptic subterranean fauna.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.