ArticleScientific reports2017
Activation of the mechanosensitive ion channel MscL by mechanical stimulation of supported Droplet-Hydrogel bilayers.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs.Advanced healthcare materials · 2024Review
- Hybrid Exb/Mot stators require substitutions distant from the chimeric pore to power flagellar rotation.Journal of bacteriology · 2024Article
- Manipulation of encapsulated artificial phospholipid membranes using sub-micellar lysolipid concentrations.Communications chemistry · 2024Article
- Tracking the Activity and Position of Mitochondrial β-Barrel Proteins.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Interaction of the mechanosensitive microswimmerRoyal Society open science · 2023Article
- Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes.International journal of molecular sciences · 2023Review
- Mechanosensitive Channel-Based Optical Membrane Tension Reporter.ACS sensors · 2023Article
- Asymmetric effects of amphipathic molecules on mechanosensitive channels.Scientific reports · 2022Article
- Enhancing membrane-based soft materials with magnetic reconfiguration events.Scientific reports · 2022Article
- Review
- Cyclodextrins increase membrane tension and are universal activators of mechanosensitive channels.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry.Chemical science · 2021Article
- Membrane protein mediated bilayer communication in networks of droplet interface bilayers.Communications chemistry · 2020Article
- The Fusion of Lipid and DNA Nanotechnology.Genes · 2019Review
- Fluorescence microscopy of piezo1 in droplet hydrogel bilayers.Channels (Austin, Tex.) · 2019Article
- OSCA/TMEM63 are an Evolutionarily Conserved Family of Mechanically Activated Ion Channels.eLife · 2018Article
- Corynebacterium glutamicum mechanosensitive channels: towards unpuzzling "glutamate efflux" for amino acid production.Biophysical reviews · 2018Review
- Reconfiguring droplet interface bilayer networks through sacrificial membranes.Biomicrofluidics · 2018Article
- Encapsulating Networks of Droplet Interface Bilayers in a Thermoreversible Organogel.Scientific reports · 2018Article
- Mechano-sensitization of mammalian neuronal networks through expression of the bacterial large-conductance mechanosensitive ion channel.Journal of cell science · 2018Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
The droplet on hydrogel bilayer (DHB) is a novel platform for investigating the function of ion channels. Advantages of this setup include tight control of all bilayer components, which is compelling for the investigation of mechanosensitive (MS) ion channels, since they are highly sensitive to their lipid environment. However, the activation of MS ion channels in planar supported lipid bilayers, such as the DHB, has not yet been established. Here we present the activation of the large conductance MS channel of E. coli, (MscL), in DHBs. By selectively stretching the droplet monolayer with nanolitre injections of buffer, we induced quantifiable DHB tension, which could be related to channel activity. The MscL activity response revealed that the droplet monolayer tension equilibrated over time, likely by insertion of lipid from solution. Our study thus establishes a method to controllably activate MS channels in DHBs and thereby advances studies of MS channels in this novel platform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.