ArticleScientific reports2017
Derivation of Functional Human Astrocytes from Cerebral Organoids.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 90 citations in OpenAlex.
- Advances in organoid imaging and automated morphometric analysis: from optical microscopy to computational approaches.Frontiers in cell and developmental biology · 2026Review
- Pluripotent stem cells-based neural organoids for modelling human brain development and diseases.Cell & bioscience · 2025Review
- Human Brain Organoids: Development and Applications.Journal of microbiology and biotechnology · 2025Review
- Inflammatory responses revealed through HIV infection of microglia-containing cerebral organoids.Journal of neuroinflammation · 2025Article
- Generating iAstrocytes From Human Induced Pluripotent Stem Cells by Combining Low-Density Passaging of Neural Progenitor Cells and Transcription Factor NFIA Transdifferentiation.Current protocols · 2024Article
- A Novel Approach to Increase Glial Cell Populations in Brain Microphysiological Systems.Advanced biology · 2024Article
- Recapitulation and investigation of human brain development with neural organoids.IBRO neuroscience reports · 2024Article
- Spatially Self-Organized Three-Dimensional Neural Concentroid as a Novel Reductionist Humanized Model to Study Neurovascular Development.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- LIF signaling regulates outer radial glial to interneuron fate during human cortical development.Cell stem cell · 2023Article
- Article
- Neural Stimulation of Brain Organoids with Dynamic Patterns: A Sentiomics Approach Directed to Regenerative Neuromedicine.NeuroSci · 2023Article
- Humanized cerebral organoids-based ischemic stroke model for discovering of potential anti-stroke agents.Acta pharmacologica Sinica · 2023Article
- Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids.Communications biology · 2023Article
- Small-molecule screen reveals pathways that regulate C4 secretion in stem cell-derived astrocytes.Stem cell reports · 2023Article
- Chemogenetic manipulation of astrocyte activity at the synapse- a gateway to manage brain disease.Frontiers in cell and developmental biology · 2023Review
- Message in a Scaffold: Natural Biomaterials for Three-Dimensional (3D) Bioprinting of Human Brain Organoids.Biomolecules · 2022Review
- Proteomic signatures of schizophrenia-sourced iPSC-derived neural cells and brain organoids are similar to patients' postmortem brains.Cell & bioscience · 2022Article
- Astrocytes derived from ASD individuals alter behavior and destabilize neuronal activity through aberrant CaMolecular psychiatry · 2022Article
- Organoid Models for Infectious Disease.Annual review of medicine · 2022Review
- The promise of organoids for unraveling the proteomic landscape of the developing human brain.Molecular psychiatry · 2022Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Astrocytes play a critical role in the development and homeostasis of the central nervous system (CNS). Astrocyte dysfunction results in several neurological and degenerative diseases. However, a major challenge to our understanding of astrocyte physiology and pathology is the restriction of studies to animal models, human post-mortem brain tissues, or samples obtained from invasive surgical procedures. Here, we report a protocol to generate human functional astrocytes from cerebral organoids derived from human pluripotent stem cells. The cellular isolation of cerebral organoids yielded cells that were morphologically and functionally like astrocytes. Immunolabelling and proteomic assays revealed that human organoid-derived astrocytes express the main astrocytic molecular markers, including glutamate transporters, specific enzymes and cytoskeletal proteins. We found that organoid-derived astrocytes strongly supported neuronal survival and neurite outgrowth and responded to ATP through transient calcium wave elevations, which are hallmarks of astrocyte physiology. Additionally, these astrocytes presented similar functional pathways to those isolated from adult human cortex by surgical procedures. This is the first study to provide proteomic and functional analyses of astrocytes isolated from human cerebral organoids. The isolation of these astrocytes holds great potential for the investigation of developmental and evolutionary features of the human brain and provides a useful approach to drug screening and neurodegenerative disease modelling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.