ArticleThe Journal of cell biology1988
Localization and mobility of gelsolin in cells.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 61 citations in OpenAlex.
- Regulation of Mitochondrial Function by the Actin Cytoskeleton.Frontiers in cell and developmental biology · 2021Review
- ATP competes with PIP2 for binding to gelsolin.PloS one · 2018Article
- Quantification of fibre polymerization through Fourier space image analysis.Proceedings. Mathematical, physical, and engineering sciences · 2011Article
- Cytosolic group IVa phospholipase A2 mediates IL-8/CXCL8-induced transmigration of human polymorphonuclear leukocytes in vitro.Journal of inflammation (London, England) · 2010Article
- VASP protects actin filaments from gelsolin: an in vitro study with implications for platelet actin reorganizations.Cell motility and the cytoskeleton · 2000Article
- Hyaluronan and the hyaluronan receptor RHAMM promote focal adhesion turnover and transient tyrosine kinase activity.The Journal of cell biology · 1994Article
- Changes in mobility of chromaffin granules in actin network with its assembly and Ca(2+)-dependent disassembly by gelsolin.Biophysical journal · 1993Article
- An actin-associated protein present in the microtubule organizing center and the growth cones of PC-12 cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1992Article
- In vivo analysis of functional domains from villin and gelsolin.The Journal of cell biology · 1992Article
- Elevation of intracellular free calcium levels in HEp-2 cells infected with enteropathogenic Escherichia coli.Infection and immunity · 1991Article
- Mbh 1: a novel gelsolin/severin-related protein which binds actin in vitro and exhibits nuclear localization in vivo.The EMBO journal · 1991Article
- Microinjection of nonmuscle and smooth muscle caldesmon into fibroblasts and muscle cells.The Journal of cell biology · 1990Article
- Leukocyte biophysics. An invited review.Cell biophysics · 1990Review
- The action of brevin, an F-actin severing protein, on the mechanical properties and ATPase activity of skinned smooth muscle.Journal of muscle research and cell motility · 1990Article
- Isolation and characterization of gelsolin from cultured BHK cells.Journal of muscle research and cell motility · 1989Article
- Association of gelsolin with actin filaments and cell membranes of macrophages and platelets.The Journal of cell biology · 1989Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
To investigate the physiologic role of gelsolin in cells, we have studied the location and mobility of gelsolin in a mouse fibroblast cell line (C3H). Gelsolin was localized by immunofluorescence of fixed and permeabilized cells and by fluorescent analog cytochemistry of living cells and cells that were fixed and/or permeabilized. Overall, the images show that in living cells gelsolin has a diffuse cytoplasmic distribution, but in fixed cells a minor fraction is associated with regions of the cell that are rich in actin filaments. The latter fraction is more prominent after permeabilization of the fixed cells because some diffuse gelsolin is not fixed and is therefore lost during permeabilization, confirmed by immunoblots. To determine quantitatively whether gelsolin is bound to actin filaments in living cells, we measured the mobility of microinjected fluorescent gelsolin by fluorescence photobleaching recovery. Gelsolin is fully mobile with a diffusion coefficient similar to that of control proteins. As a positive control, fluorescent phalloidin, which binds actin filaments, is totally immobile. These results are supported by immunoblots on cells permeabilized with detergent. All the endogenous gelsolin is extracted, and the half-time for the extraction is approximately 5 s, which is about the rate predicted for diffusion. Therefore, gelsolin is not tightly bound to actin filaments in cells. The most likely interpretation of the difference between living and fixed cells is that fixation traps a fraction of gelsolin that is associated with actin filaments in short-lived complexes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.