ArticleStem cell reports2017
A Genome-wide Analysis of Human Pluripotent Stem Cell-Derived Endothelial Cells in 2D or 3D Culture.
Article in Stem cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 49 citations in OpenAlex.
- Synovial fibroblasts modulate endothelial activation in an acute injury-on-a-chip model.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Endothelial cell supplementation promotes xenograft revascularization during short-term ovarian tissue transplantation.Bioactive materials · 2025Article
- Extracellular-Matrix-Mimetic Hydrogels by Using Nanomaterials.International journal of molecular sciences · 2025Review
- The Rise of Pluripotent Stem Cell-Derived Glia Models of Neuroinflammation.Neurology international · 2025Review
- Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells.APL bioengineering · 2023Article
- The Differentiation of Pluripotent Stem Cells towards Endothelial Progenitor Cells - Potential Application in Pulmonary Arterial Hypertension.International journal of stem cells · 2022Article
- Vascularizing the brainiScience · 2022Review
- Drivers of transcriptional variance in human intestinal epithelial organoids.Physiological genomics · 2021Article
- High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology.Chemical reviews · 2021Review
- A comprehensive library of human transcription factors for cell fate engineering.Nature biotechnology · 2021Article
- A protocol for rapid pericyte differentiation of human induced pluripotent stem cells.STAR protocols · 2021Article
- Comparative Study of Human Pluripotent Stem Cell-Derived Endothelial Cells in Hydrogel-Based Culture Systems.ACS omega · 2021Article
- Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Recent progress and new challenges in modeling of human pluripotent stem cell-derived blood-brain barrier.Theranostics · 2021Review
- Organ-on-a-chip: the next generation platform for risk assessment of radiobiology.RSC advances · 2020Review
- Article
- From Human Pluripotent Stem Cells to 3D Cardiac Microtissues: Progress, Applications and Challenges.Bioengineering (Basel, Switzerland) · 2020Review
- Sustained release and protein stabilization reduce the growth factor dosage required for human pluripotent stem cell expansion.Biomaterials · 2020Article
- Evolutionarily conserved sequence motif analysis guides development of chemically defined hydrogels for therapeutic vascularization.Science advances · 2020Article
- Synthetic alternatives to Matrigel.Nature reviews. Materials · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 3 institutions in 1 country.
Funding
Abstract
A defined protocol for efficiently deriving endothelial cells from human pluripotent stem cells was established and vascular morphogenesis was used as a model system to understand how synthetic hydrogels influence global biological function compared with common 2D and 3D culture platforms. RNA sequencing demonstrated that gene expression profiles were similar for endothelial cells and pericytes cocultured in polyethylene glycol (PEG) hydrogels or Matrigel, while monoculture comparisons identified distinct vascular signatures for each cell type. Endothelial cells cultured on tissue-culture polystyrene adopted a proliferative phenotype compared with cells cultured on or encapsulated in PEG hydrogels. The proliferative phenotype correlated to increased FAK-ERK activity, and knockdown or inhibition of ERK signaling reduced proliferation and expression for cell-cycle genes while increasing expression for "3D-like" vasculature development genes. Our results provide insight into the influence of 2D and 3D culture formats on global biological processes that regulate cell function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.