ArticleG3 (Bethesda, Md.)2017
From Pine Cones to Read Clouds: Rescaffolding the Megagenome of Sugar Pine (
Article in G3 (Bethesda, Md.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Transcriptomic and enzymological evidence for plastid peptidoglycan synthesis in the gymnosperm Picea abies.The Plant journal : for cell and molecular biology · 2025Article
- A genome sequence for the threatened whitebark pine.G3 (Bethesda, Md.) · 2024Article
- Article
- Identification and high-throughput genotyping of single nucleotide polymorphism markers in a non-model conifer (Abies nordmanniana (Steven) Spach).Scientific reports · 2023Article
- Growth, drought response, and climate-associated genomic structure in whitebark pine in the Sierra Nevada of California.Ecology and evolution · 2023Article
- Standardized genome-wide function prediction enables comparative functional genomics: a new application area for Gene Ontologies in plants.GigaScience · 2022Article
- Comparative Study of Pine Reference Genomes Reveals Transposable Element Interconnected Gene Networks.Genes · 2020Article
- Genomic basis of white pine blister rust quantitative disease resistance and its relationship with qualitative resistance.The Plant journal : for cell and molecular biology · 2020Article
- Molecular study of drought response in the Mediterranean coniferEcology and evolution · 2020Article
- Genome-Wide Variant Identification and High-Density Genetic Map Construction Using RADseq forG3 (Bethesda, Md.) · 2019Article
- A Reference Genome Sequence for the European Silver Fir (G3 (Bethesda, Md.) · 2019Article
- Linked read technology for assembling large complex and polyploid genomes.BMC genomics · 2018Article
- Comparative Transcriptomics Among Four White Pine Species.G3 (Bethesda, Md.) · 2018Article
- Independent assessment and improvement of wheat genome sequence assemblies using Fosill jumping libraries.GigaScience · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigate the utility and scalability of new read cloud technologies to improve the draft genome assemblies of the colossal, and largely repetitive, genomes of conifers. Synthetic long read technologies have existed in various forms as a means of reducing complexity and resolving repeats since the outset of genome assembly. Recently, technologies that combine subhaploid pools of high molecular weight DNA with barcoding on a massive scale have brought new efficiencies to sample preparation and data generation. When combined with inexpensive light shotgun sequencing, the resulting data can be used to scaffold large genomes. The protocol is efficient enough to consider routinely for even the largest genomes. Conifers represent the largest reference genome projects executed to date. The largest of these is that of the conifer
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.