ArticleGenome medicine2017
DNA methylation changes at infertility genes in newborn twins conceived by in vitro fertilisation.
Article in Genome medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.
- Association of medically assisted reproduction with offspring cord blood DNA methylation across cohorts.Human reproduction (Oxford, England) · 2021Pooled it
- DNA methylation profiles and cancer in children conceived after assisted reproductive technology.Clinical epigenetics · 2025Article
- ART altered DNA methylation of the imprinted gene H19 in fetal tissue after multifetal pregnancy reduction.Journal of assisted reproduction and genetics · 2024Article
- The Role of One-Carbon Metabolism and Methyl Donors in Medically Assisted Reproduction: A Narrative Review of the Literature.International journal of molecular sciences · 2024Review
- Trophectoderm biopsy of blastocysts following IVF and embryo culture increases epigenetic dysregulation in a mouse model.Human reproduction (Oxford, England) · 2024Article
- Capturing sex-specific and hypofertility-linked effects of assisted reproductive technologies on the cord blood DNA methylome.Clinical epigenetics · 2023Article
- The X-factor in ART: does the use of assisted reproductive technologies influence DNA methylation on the X chromosome?Human genomics · 2023Article
- Genome-wide assessment of DNA methylation alterations induced by superovulation, sexual immaturity and in vitro follicle growth in mouse blastocysts.Clinical epigenetics · 2023Article
- A Paternal Methylation Error in the Congenital Hydrocephalic Texas (H-Tx) Rat Is Partially Rescued with Natural Folate Supplements.International journal of molecular sciences · 2023Article
- DNA Methylation in Offspring Conceived after Assisted Reproductive Techniques: A Systematic Review and Meta-Analysis.Journal of clinical medicine · 2022Review
- Epigenetic Risks of Medically Assisted Reproduction.Journal of clinical medicine · 2022Review
- Contemporary Use of ICSI and Epigenetic Risks to Future Generations.Journal of clinical medicine · 2022Review
- DNA methylation in newborns conceived by assisted reproductive technology.Nature communications · 2022Article
- Genome-Wide Analysis of DNA Methylation in Buccal Cells of Children Conceived through IVF and ICSI.Genes · 2021Article
- Conception by fertility treatment and offspring deoxyribonucleic acid methylation.Fertility and sterility · 2021Article
- Whole-Genome Methylation Analysis Revealed ART-Specific DNA Methylation Pattern of Neuro- and Immune-System Pathways in Chinese Human Neonates.Frontiers in genetics · 2021Article
- DNA methylation differences at birth after conception through ART.Human reproduction (Oxford, England) · 2021Article
- Integrated multi-omics reveal epigenomic disturbance of assisted reproductive technologies in human offspring.EBioMedicine · 2020Article
- Contributions to prematurity of maternal health conditions, subfertility, and assisted reproductive technology.Fertility and sterility · 2020Article
- 18q12.3-q21.1 microdeletion detected in the prenatally alcohol-exposed dizygotic twin with discordant fetal alcohol syndrome phenotype.Molecular genetics & genomic medicine · 2020Article
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 5 countries.
Funding
Abstract
backgroundThe association of in vitro fertilisation (IVF) and DNA methylation has been studied predominantly at regulatory regions of imprinted genes and at just thousands of the ~28 million CpG sites in the human genome.
methodsWe investigated the links between IVF and DNA methylation patterns in whole cord blood cells (n = 98) and cord blood mononuclear cells (n = 82) from newborn twins using genome-wide methylated DNA immunoprecipitation coupled with deep sequencing.
resultsAt a false discovery rate (FDR) of 5%, we identified one significant whole blood DNA methylation change linked to conception via IVF, which was located ~3 kb upstream of TNP1, a gene previously linked to male infertility. The 46 most strongly associated signals (FDR of 25%) included a second region in a gene also previously linked to infertility, C9orf3, suggesting that our findings may in part capture the effect of parental subfertility. Using twin modelling, we observed that individual-specific environmental factors appear to be the main overall contributors of methylation variability at the FDR 25% IVF-associated differentially methylated regions, although evidence for methylation heritability was also obtained at several of these regions. We replicated previous findings of differential methylation associated with IVF at the H19/IGF2 region in cord blood mononuclear cells, and we validated the signal at C9orf3 in monozygotic twins. We also explored the impact of intracytoplasmic sperm injection on the FDR 25% signals for potential effects specific to male or female infertility factors.
conclusionsTo our knowledge, this is the most comprehensive study of DNA methylation profiles at birth and IVF conception to date, and our results show evidence for epigenetic modifications that may in part reflect parental subfertility.
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