Evidence map›Paper›PMID 28340575›Full record

ArticleJournal of neuroinflammation2017

NOX2 deficiency alters macrophage phenotype through an IL-10/STAT3 dependent mechanism: implications for traumatic brain injury.

James P Barrett, Rebecca J Henry, Sonia Villapol, Bogdan A Stoica, Alok Kumar, Mark P Burns, Alan I Faden, David J Loane

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed, 93 citations in OpenAlex.

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  4. Macrophages on demand: How tissue trauma shapes their role.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

James P BarrettDepartment of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA.
Rebecca J HenryDepartment of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA.
Sonia VillapolLaboratory for Brain Injury and Dementia, Department of Neuroscience, Georgetown University, Washington, DC, USA.
Bogdan A StoicaDepartment of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA.
Alok KumarDepartment of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA.
Mark P BurnsLaboratory for Brain Injury and Dementia, Department of Neuroscience, Georgetown University, Washington, DC, USA.
Alan I FadenDepartment of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA.
David J LoaneDepartment of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA. dloane@anes.umm.edu.
University of Maryland, Baltimore · USGeorgetown University · US

Funding

University of Maryland Claude D. Pepper Older Americans Independence Center (UM-OAIC)P30AG028747 · NIA · UNIVERSITY OF MARYLAND BALTIMORE · PI ALICE S. RYAN · 2006 to 2026
$28.9M
MODULATION OF INJURY BY METABOTROPIC GLUTAMATE RECEPTORSR01NS037313 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI FADEN, ALAN IRA · 1999 to 2019
$5.8M
Cognitive and Neuroimaging Study in Sickle Cell DiseaseR01NS046717 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI MELHEM, ELIAS R · 2004 to 2007
$2.1M
Microglial activation phenotypes and mechanisms of repair in the aged TBI brainR01NS082308 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI LOANE, DAVID J. · 2013 to 2016
$1.4M
The role of Abeta in injury-induced cell deathR03NS067417 · NINDS · GEORGETOWN UNIVERSITY · PI BURNS, MARK P · 2010 to 2011
$152k
NIA NIH HHS P30 AG028747NINDS NIH HHS R01 NS037313NINDS NIH HHS R01 NS046717NINDS NIH HHS R01 NS082308NINDS NIH HHS R03 NS067417
6 · The paper itself

Abstract

backgroundNADPH oxidase (NOX2) is an enzyme system that generates reactive oxygen species (ROS) in microglia and macrophages. Excessive ROS production is linked with neuroinflammation and chronic neurodegeneration following traumatic brain injury (TBI). Redox signaling regulates macrophage/microglial phenotypic responses (pro-inflammatory versus anti-inflammatory), and NOX2 inhibition following moderate-to-severe TBI markedly reduces pro-inflammatory activation of macrophages/microglia resulting in concomitant increases in anti-inflammatory responses. Here, we report the signaling pathways that regulate NOX2-dependent macrophage/microglial phenotype switching in the TBI brain.

methodsBone marrow-derived macrophages (BMDMs) prepared from wildtype (C57Bl/6) and NOX2 deficient (NOX2

resultsUsing an LPS/IL-4-stimulated BMDM model that mimics the mixed pro- and anti-inflammatory responses observed in the injured cortex, we show that NOX2

conclusionsThese studies indicate that following TBI NOX2 inhibition promotes a robust anti-inflammatory response in macrophages/microglia that is mediated by the IL-10/STAT3 signaling pathway. Thus, therapeutic interventions that inhibit macrophage/microglial NOX2 activity may improve TBI outcomes by not only limiting pro-inflammatory neurotoxic responses, but also enhancing IL-10-mediated anti-inflammatory responses that are neuroprotective.

Indexed as

AnimalsBrain Injuries, TraumaticInflammationInterleukin-10Macrophage ActivationMacrophagesMaleMiceMice, Inbred C57BLMice, KnockoutNADPH Oxidase 2PhenotypeSTAT3 Transcription FactorCybb protein, mouseIL10 protein, mouseInterleukin-10NADPH Oxidase 2Stat3 protein, mouseSTAT3 Transcription FactorInterleukin-10MacrophageNADPH oxidaseNeuroinflammationNOX2Traumatic brain injury

Identifiers

PMID28340575
PMCPMC5366128
OpenAlexW2600321955

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.