ArticleBMC infectious diseases2017
A novel real-time PCR assay for specific detection of Brucella melitensis.
Article in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- A novel duplex TaqMan qPCR assay for rapid differential diagnosis and co-infection screening of Orf virus and Brucella spp. in goats.Veterinary research communications · 2026Article
- Evaluation of AI-enhanced FTIR spectroscopy for species and biovar typing of intracellular pathogenic Brucella spp.BMC microbiology · 2026Article
- Two novel real-time PCR assays forMicrobiology spectrum · 2026Article
- Development of a rapid and sensitive RPA-CRISPR/Cas12a-based assay for the detection ofMicrobiology spectrum · 2025Article
- Epidemiological analysis of human brucellosis in North Khorasan province, Iran (2018-2023): a six-year multicenter retrospective study.BMC infectious diseases · 2025Article
- One-pot, uracil-DNA-glycosylase-aided, and multi-indicator nanobiosensor detection platform for identifying Brucella melitensis from the Brucella spp.BMC microbiology · 2025Article
- Development of a colloidal gold immunochromatographic test strip for detecting the smooth Brucella.Scientific reports · 2024Article
- Evidence-practice gap analysis in the role of tick in brucellosis transmission: a scoping review.Infectious diseases of poverty · 2024Article
- CRISPR-Cas12a test strip (CRISPR/CAST) package: In-situ detection of Brucella from infected livestock.BMC veterinary research · 2023Article
- A Challenging Diagnosis of Febrile Pancytopenia in a Patient With a History of Autoimmune Disease.Cureus · 2023Article
- Serological and molecular prevalence ofFrontiers in veterinary science · 2023Article
- Prevalence of brucellosis in livestock keepers and domestic ruminants in Baringo County, Kenya.PLOS global public health · 2022Article
- Article
- Case Report: Metagenomic Next-Generation Sequencing Confirmed a Case of Central Nervous System Infection WithFrontiers in medicine · 2021Article
- Seroprevalence of brucellosis in sheep and goats in the Arabian Gulf region.Veterinary world · 2020Article
- Optimization and validation of a real-time polymerase chain reaction protocol for the diagnosis of human brucellosis.Folia microbiologica · 2020Article
- Specific Detection and Differentiation BetweenFrontiers in veterinary science · 2020Article
- Development of a DNA aptamer to detectIranian journal of veterinary research · 2020Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- Whole Genome Sequencing for Tracing Geographical Origin of Imported Cases of Human Brucellosis in Sweden.Microorganisms · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBrucellosis is a zoonosis that occurs worldwide. The disease has been completely eradicated in livestock in Sweden in 1994, and all cases of confirmed human brucellosis are imported into Sweden from other countries. However, due to an increase in the number of refugees and asylum seekers from the middle-east to Sweden, there is a need to improve the current diagnostic methodology for Brucella melitensis. Whilst culture of Brucella species can be used as a diagnostic tool, real-time PCR approaches provide a much faster result. The aim of this study was to set up a species-specific real-time PCR for the detection of all biovars of Brucella melitensis, which could be used routinely in diagnostic laboratories.
methodsA Brucella melitensis real-time PCR assay was designed using all available genomes in the public database of Brucella (N = 96) including all complete genomes of Brucella melitensis (N = 17). The assay was validated with a collection of 37 Brucella species reference strains, 120 Brucella melitensis human clinical isolates, and 45 clinically relevant non-Brucella melitensis strains.
resultsIn this study we developed a single real-time PCR for the specific detection of all biovars of Brucella melitensis.
conclusionsThis new real-time PCR method shows a high specificity (100%) and a high sensitivity (1.25 GE/μl) and has been implemented in the laboratories of four governmental authorities across Sweden.
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