Evidence map›Paper›PMID 28339869›Full record

ArticleAlcohol and alcoholism (Oxford, Oxfordshire)2017

Alcohol Induces Cellular Senescence and Impairs Osteogenic Potential in Bone Marrow-Derived Mesenchymal Stem Cells.

Xi Chen, Mao Li, Jinku Yan, Tao Liu, Guoqing Pan, Huilin Yang, Ming Pei, Fan He

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Alcohol and alcoholism (Oxford, Oxfordshire), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed, 2 pooled it
2.8field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 2 syntheses or guidelines pooled it, 53 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Trial
  4. Review
  5. Mitochondrial regulation of cellular senescence heterogeneity.Frontiers in cell and developmental biology · 2026
    Review
  6. Review
  7. Review
  8. Article
  9. Review
  10. Emerging role of liver-bone axis in osteoporosis.Journal of orthopaedic translation · 2024
    Review
  11. Article
  12. Article
  13. Article
  14. Musculoskeletal and Psychological Rehabilitation.Indian journal of orthopaedics · 2023
    Review
  15. Article
  16. Article
  17. Article
  18. Review
  19. [Progress of pathogenesis and genetics of alcohol-induced osteonecrosis of femoral head].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2022
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Xi ChenDepartment of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou 215153, Jiangsu, China.
Mao LiDepartment of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou 215153, Jiangsu, China.
Jinku YanDepartment of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou 215153, Jiangsu, China.
Tao LiuDepartment of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou 215153, Jiangsu, China.
Guoqing PanDepartment of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou 215153, Jiangsu, China.
Huilin YangDepartment of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou 215153, Jiangsu, China.
Ming PeiStem Cell and Tissue Engineering Laboratory, Department of Orthopaedics and Division of Exercise Physiology, West Virginia University, PO Box 9196, One Medical Center Drive, Morgantown, WV 26505-9196, USA.
Fan HeDepartment of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou 215153, Jiangsu, China.
Soochow University · CNFirst Affiliated Hospital of Soochow University · CNWest Virginia University · US

Funding

Decellularized Matrix and Cartilage RegenerationR01AR067747 · NIAMS · WEST VIRGINIA UNIVERSITY · PI PEI, MING · 2016 to 2020
$1.7M
Decellularized Stem Cell Matrix Rejuvenates Human Cells from Herniated DiscsR03AR062763 · NIAMS · WEST VIRGINIA UNIVERSITY · PI PEI, MING · 2013 to 2015
$222k
NIAMS NIH HHS R01 AR067747NIAMS NIH HHS R03 AR062763
6 · The paper itself

Abstract

aimsChronic and excessive alcohol consumption is a high-risk factor for osteoporosis. Bone marrow-derived mesenchymal stem cells (BM-MSCs) play an important role in bone formation; however, they are vulnerable to ethanol (EtOH). The purpose of this research was to investigate whether EtOH could induce premature senescence in BM-MSCs and subsequently impair their osteogenic potential.

methodsHuman BM-MSCs were exposed to EtOH ranging from 10 to 250 mM. Senescence-associated β-galactosidase (SA-β-gal) activity, cell cycle distribution, cell proliferation and reactive oxygen species (ROS) were evaluated. Mineralization and osteoblast-specific gene expression were evaluated during osteogenesis in EtOH-treated BM-MSCs. To investigate the role of silent information regulator Type 1 (SIRT1) in EtOH-induced senescence, resveratrol (ResV) was used to activate SIRT1 in EtOH-treated BM-MSCs.

resultsEtOH treatments resulted in senescence-associated phenotypes in BM-MSCs, such as decreased cell proliferation, increased SA-β-gal activity and G0/G1 cell cycle arrest. EtOH also increased the intracellular ROS and the expression of senescence-related genes, such as p16INK4α and p21. The down-regulated levels of SIRT1 accompanied with suppressed osteogenic differentiation were confirmed in EtOH-treated BM-MSCs. Activation of SIRT1 by ResV partially counteracted the effects of EtOH by decreasing senescence markers and rescuing the inhibited osteogenesis.

conclusionEtOH treatments induced premature senescence in BM-MSCs in a dose-dependent manner that was responsible for EtOH-impaired osteogenic differentiation. Activation of SIRT1 was effective in ameliorating EtOH-induced senescence phenotypes in BMSCs and could potentially lead to a new strategy for clinically preventing or treating alcohol-induced osteoporosis. SHORT SUMMARY: Ethanol (EtOH) treatments induce premature senescence in marrow-derived mesenchymal stem cells in a dose-dependent manner that is responsible for EtOH-impaired osteogenic differentiation. Activation of SIRT1 is effective in ameliorating EtOH-induced senescence phenotypes, which potentially leads to a new strategy for clinically treating alcohol-induced osteoporosis.

Indexed as

Cell DifferentiationCells, CulturedCellular SenescenceDose-Response Relationship, DrugEthanolHumansMesenchymal Stem CellsOsteogenesisEthanol

Identifiers

PMID28339869
PMCPMC5397879
OpenAlexW2606769691

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.