ArticlePloS one2017
A high-throughput qPCR system for simultaneous quantitative detection of dairy Lactococcus lactis and Leuconostoc bacteriophages.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Functional Evaluation of a Conserved HNH Endonuclease Gene in Lactococcal Skunavirus Genomes.Microbial biotechnology · 2026Article
- Development and validation of a LAMP assay for detection of genetically diverseFrontiers in microbiology · 2026Article
- Distinct shifts in bacteriophage diversity and abundance during various stages of Gouda-type cheese production.Applied and environmental microbiology · 2025Article
- Functional and practical insights into three lactococcal antiphage systems.Applied and environmental microbiology · 2024Article
- CRISPR-Cas provides limited phage immunity to a prevalent gut bacterium in gnotobiotic mice.The ISME journal · 2023Article
- High-throughput qPCR and 16S rRNA gene amplicon sequencing as complementary methods for the investigation of the cheese microbiota.BMC microbiology · 2022Article
- Development of a High-Throughput Microfluidic qPCR System for the Quantitative Determination of Quality-Relevant Bacteria in Cheese.Frontiers in microbiology · 2020Article
- Article
- A Protocol for Extraction of Infective Viromes Suitable for Metagenomics Sequencing from Low Volume Fecal Samples.Viruses · 2019Article
- Unprecedented Diversity of Lactococcal Group 936 Bacteriophages Revealed by Amplicon Sequencing of the Portal Protein Gene.Viruses · 2019Article
- Rates of Mutation and Recombination in Siphoviridae Phage Genome Evolution over Three Decades.Molecular biology and evolution · 2018Article
- Real-Time qPCR as a Method for Detection of Antibody-Neutralized Phage Particles.Frontiers in microbiology · 2017Article
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Authors and funding
4 authors.
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Abstract
Simultaneous quantitative detection of Lactococcus (Lc.) lactis and Leuconostoc species bacteriophages (phages) has not been reported in dairies using undefined mixed-strain DL-starters, probably due to the lack of applicable methods. We optimized a high-throughput qPCR system that allows simultaneous quantitative detection of Lc. lactis 936 (now SK1virus), P335, c2 (now C2virus) and Leuconostoc phage groups. Component assays are designed to have high efficiencies and nearly the same dynamic detection ranges, i.e., from ~1.1 x 105 to ~1.1 x 101 phage genomes per reaction, which corresponds to ~9 x 107 to ~9 x 103 phage particles mL-1 without any additional up-concentrating steps. The amplification efficiencies of the corresponding assays were 100.1±2.6, 98.7±2.3, 101.0±2.3 and 96.2±6.2. The qPCR system was tested on samples obtained from a dairy plant that employed traditional mother-bulk-cheese vat system. High levels of 936 and P335 phages were detected in the mother culture and the bulk starter, but also in the whey samples. Low levels of phages were detected in the cheese milk samples.
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