Evidence map›Paper›PMID 28339303›Full record

ArticleMonoclonal antibodies in immunodiagnosis and immunotherapy2017

Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.

Yuki Fujii, Mika K Kaneko, Satoshi Ogasawara, Shinji Yamada, Miyuki Yanaka, Takuro Nakamura, Noriko Saidoh, Kanae Yoshida, Ryusuke Honma, Yukinari Kato

Abstract read
In one paragraph

Article in Monoclonal antibodies in immunodiagnosis and immunotherapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

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  18. HMonoclonal antibodies in immunodiagnosis and immunotherapy · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuki Fujii1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Mika K Kaneko1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Satoshi Ogasawara1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Shinji Yamada1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Miyuki Yanaka1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Takuro Nakamura1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Noriko Saidoh1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Kanae Yoshida1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Ryusuke Honma1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Yukinari Kato1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.

Indexed as

AnimalsAntibodies, MonoclonalAntibody AffinityAntibody SpecificityCHO CellsCricetulusErbB ReceptorsGene ExpressionMembrane GlycoproteinsMicePeptidesPodoplaninRatsStaining and LabelingAntibodies, MonoclonalErbB ReceptorsMembrane GlycoproteinsPdpn protein, ratPeptidesPodoplaninaffinity tagmonoclonal antibodypodoplaninprotein purificationRAP tag

Identifiers

PMID28339303
PMCPMC5404252

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.