Evidence map›Paper›PMID 28337708›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation.

Yun Chen, Chuan Li, Ya-Hui Tsai, Sheng-Hong Tseng

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yun ChenDepartment of Surgery, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei, Taiwan.
Chuan LiDepartment of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan.
Ya-Hui TsaiDepartment of Surgery, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei, Taiwan. yahuitsi@gmail.com.
Sheng-Hong TsengDepartment of Surgery, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan. tsh5110@ntu.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The isolation and culture of intestinal stem cells (ISCs) was first demonstrated in the very recent decade with the identification of ISC marker Lgr5. The growth of ISCs into crypt organoids provides an in vitro model for studying the mucosal physiology, intestinal cancer tumorigenesis, and intestinal regeneration. Here, we describe two different isolation protocols and demonstrate a fixation method that aids in the confocal observation of the organoids.

Indexed as

Cell Culture TechniquesCells, CulturedCell SeparationHumansIntestinesIn Vitro TechniquesMicroscopy, ConfocalOrganoidsStem CellsConfocal observationCrypt organoidIntestinal stem cell (ISC)IsolationThree-dimensional imaging

Identifiers

PMID28337708

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.