ArticleBioMed research international2017
Rapid Chondrocyte Isolation for Tissue Engineering Applications: The Effect of Enzyme Concentration and Temporal Exposure on the Matrix Forming Capacity of Nasal Derived Chondrocytes.
Article in BioMed research international, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Effects of the space environment on articular cartilage homeostasis: a review.NPJ microgravity · 2026Review
- Chondrocytes supplemented to bone graft-containing scaffolds expedite cranial defect repair.Scientific reports · 2023Article
- Harmonization and standardization of nucleus pulposus cell extraction and culture methods.JOR spine · 2023Article
- In Vitro and Ectopic In Vivo Studies toward the Utilization ofInternational journal of molecular sciences · 2022Article
- Pericellular heparan sulfate proteoglycans: Role in regulating the biosynthetic response of nucleus pulposus cells to osmotic loading.JOR spine · 2022Article
- A high-resolution route map reveals distinct stages of chondrocyte dedifferentiation for cartilage regeneration.Bone research · 2022Article
- The Endplate Role in Degenerative Disc Disease Research: The Isolation of Human Chondrocytes from Vertebral Endplate-An Optimised Protocol.Bioengineering (Basel, Switzerland) · 2022Article
- Optimization of Protocol for Isolation of Chondrocytes from Human Articular Cartilage.Cartilage · 2021Article
- Article
- Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses.Scientific reports · 2021Article
- Human nasal septal chondrocytes (NSCs) preconditioned on NSC-derived matrix improve their chondrogenic potential.Biomaterials research · 2021Article
- Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.International journal of molecular sciences · 2020Article
- Tissue engineering applications in otolaryngology-The state of translation.Laryngoscope investigative otolaryngology · 2020Review
- Neural Crest-Derived Chondrocytes Isolation for Tissue Engineering in Regenerative Medicine.Cells · 2020Article
- Where is human-based cellular pharmaceutical R&D taking us in cartilage regeneration?3 Biotech · 2020Review
- Enzyme Development for Human Islet Isolation: Five Decades of Progress or Stagnation?The review of diabetic studies : RDS · 2017Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laboratory based processing and expansion to yield adequate cell numbers had been the standard in Autologous Disc Chondrocyte Transplantation (ADCT), Allogeneic Juvenile Chondrocyte Implantation (NuQu®), and Matrix-Induced Autologous Chondrocyte Implantation (MACI). Optimizing cell isolation is a key challenge in terms of obtaining adequate cell numbers while maintaining a vibrant cell population capable of subsequent proliferation and matrix elaboration. However, typical cell yields from a cartilage digest are highly variable between donors and based on user competency. The overall objective of this study was to optimize chondrocyte isolation from cartilaginous nasal tissue through modulation of enzyme concentration exposure (750 and 3000 U/ml) and incubation time (1 and 12 h), combined with physical agitation cycles, and to assess subsequent cell viability and matrix forming capacity. Overall, increasing enzyme exposure time was found to be more detrimental than collagenase concentration for subsequent viability, proliferation, and matrix forming capacity (sGAG and collagen) of these cells resulting in nonuniform cartilaginous matrix deposition. Taken together, consolidating a 3000 U/ml collagenase digest of 1 h at a ratio of 10 ml/g of cartilage tissue with physical agitation cycles can improve efficiency of chondrocyte isolation, yielding robust, more uniform matrix formation.
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