ArticleExperimental eye research2017
The role of pili in Bacillus cereus intraocular infection.
Article in Experimental eye research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 27 citations in OpenAlex.
- Characterization of the pathogenicity of aVirulence · 2022Article
- Roles of CCL2 and CCL3 in intraocular inflammation during Bacillus endophthalmitis.Experimental eye research · 2022Article
- INCIDENCE AND RISK FACTORS OF POSTOPERATIVE ENDOPHTHALMITIS AFTER PRIMARY SURGICAL REPAIR COMBINED WITH INTRAOCULAR FOREIGN BODY REMOVAL.Retina (Philadelphia, Pa.) · 2022Article
- C-X-C Chemokines Influence Intraocular Inflammation During Bacillus Endophthalmitis.Investigative ophthalmology & visual science · 2021Article
- Immune Inhibitor A Metalloproteases Contribute to Virulence inInfection and immunity · 2021Article
- TheMicrobiology (Reading, England) · 2021Article
- Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis.Journal of visualized experiments : JoVE · 2021Article
- The Food Poisoning Toxins ofToxins · 2021Review
- TheToxins · 2020Review
- Prevalence of antibiotic-resistant, toxic metal-tolerant and biofilm-forming bacteria in hospital surroundings.Environmental analysis, health and toxicology · 2020Article
- Expression ofMicroorganisms · 2020Article
- The cereus matter of Bacillus endophthalmitis.Experimental eye research · 2020Review
- Article
- S-layer Impacts the Virulence of Bacillus in Endophthalmitis.Investigative ophthalmology & visual science · 2019Article
- Ocular Glands Become Infected Secondarily to Infectious Keratitis and Play a Role in Corneal Resistance to Infection.Journal of virology · 2019Article
- Antimicrobial Activity of Essential Oil ofFrontiers in plant science · 2019Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Bacterial endophthalmitis is a potentially blinding intraocular infection. The bacterium Bacillus cereus causes a devastating form of this disease which progresses rapidly, resulting in significant inflammation and loss of vision within a few days. The outer surface of B. cereus incites the intraocular inflammatory response, likely through interactions with innate immune receptors such as TLRs. This study analyzed the role of B. cereus pili, adhesion appendages located on the bacterial surface, in experimental endophthalmitis. To test the hypothesis that the presence of pili contributed to intraocular inflammation and virulence, we analyzed the progress of experimental endophthalmitis in mouse eyes infected with wild type B. cereus (ATCC 14579) or its isogenic pilus-deficient mutant (ΔbcpA-srtD-bcpB or ΔPil). One hundred CFU were injected into the mid-vitreous of one eye of each mouse. Infections were analyzed by quantifying intraocular bacilli and retinal function loss, and by histology from 0 to 12 h postinfection. In vitro growth and hemolytic phenotypes of the infecting strains were also compared. There was no difference in hemolytic activity (1:8 titer), motility, or in vitro growth (p > 0.05, every 2 h, 0-18 h) between wild type B. cereus and the ΔPil mutant. However, infected eyes contained greater numbers of wild type B. cereus than ΔPil during the infection course (p ≤ 0.05, 3-12 h). Eyes infected with wild type B. cereus experienced greater losses in retinal function than eyes infected with the ΔPil mutant, but the differences were not always significant. Eyes infected with ΔPil or wild type B. cereus achieved similar degrees of severe inflammation. The results indicated that the intraocular growth of pilus-deficient B. cereus may have been better controlled, leading to a trend of greater retinal function in eyes infected with the pilus-deficient strain. Although this difference was not enough to significantly alter the severity of the inflammatory response, these results suggest a potential role for pili in protecting B. cereus from clearance during the early stages of endophthalmitis, which is a newly described virulence mechanism for this organism and this infection.
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