ArticleMethods in enzymology2017
Probing Cdc42 Polarization Dynamics in Budding Yeast Using a Biosensor.
Article in Methods in enzymology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Patterning of the cell cortex by Rho GTPases.Nature reviews. Molecular cell biology · 2024Review
- Cdc42 couples septin recruitment to the axial landmark assembly via Axl2 in budding yeast.Journal of cell science · 2024Article
- Regulation of Cdc42 protein turnover modulates the filamentous growth MAPK pathway.The Journal of cell biology · 2022Article
- Up-regulation of the Cdc42 GTPase limits the replicative life span of budding yeast.Molecular biology of the cell · 2022Article
- Spatiotemporal control of pathway sensors and cross-pathway feedback regulate a differentiation MAPK pathway in yeast.Journal of cell science · 2021Article
- Essential role of the endocytic site-associated protein Ecm25 in stress-induced cell elongation.Cell reports · 2021Article
- Regulation of Cdc42 for polarized growth in budding yeast.Microbial cell (Graz, Austria) · 2020Review
- Regulation of intrinsic polarity establishment by a differentiation-type MAPK pathway inJournal of cell science · 2020Article
- The adaptor protein Ste50 directly modulates yeast MAPK signaling specificity through differential connections of its RA domain.Molecular biology of the cell · 2019Article
- Autoactivation of small GTPases by the GEF-effector positive feedback modules.F1000Research · 2019Review
- The shared role of the Rsr1 GTPase and Gic1/Gic2 in Cdc42 polarization.Molecular biology of the cell · 2018Article
- Directly light-regulated binding of RGS-LOV photoreceptors to anionic membrane phospholipids.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Fine-tuning the orientation of the polarity axis by Rga1, a Cdc42 GTPase-activating protein.Molecular biology of the cell · 2017Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Cdc42 is a small guanosine triphosphatase (GTPase) that plays a central role in polarity development in diverse cell types. Since the activity of Cdc42 is dynamically controlled in time and space, it is required to develop a biosensor to monitor its activation in vivo. In this chapter, we describe the construction and usage of a simple and robust biosensor for monitoring active Cdc42 in budding yeast. This affinity-based biosensor uses a red fluorescent protein fused to a Cdc42- and Rac-interactive binding motif from one of the Cdc42 effector proteins. Because it binds specifically to the GTP-bound Cdc42, this biosensor can be used to monitor Cdc42 activation in vivo. This or similar biosensors can be widely used for studying GTPase signaling in other cell types because of the conserved CRIB motif present among GTPase targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.