ArticleMicrobiome2017
Potential for hydrogen-oxidizing chemolithoautotrophic and diazotrophic populations to initiate biofilm formation in oligotrophic, deep terrestrial subsurface waters.
Article in Microbiome, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- Groundwater microbial communities across the terrestrial subsurface.Nature microbiology · 2026Review
- Physiological responses to nitrogen availability inMicrobiology spectrum · 2026Article
- Multi-Year Biofilm Formation on Granitic Surfaces Reveals Dynamic Microbial Communities in Fennoscandian Shield Deep Groundwaters.Microbial ecology · 2026Article
- Exploring microbial diversity using cell-size fractionated enrichment incubations from subsurface aquifers at Äspö, Sweden.Communications biology · 2026Article
- Two worlds beneath: Distinct microbial strategies of the rock-attached and planktonic subsurface biosphere.Microbiome · 2026Article
- Site-specific incubations reveal biofilm diversity and functional adaptations in deep, ancient desert aquifers.Frontiers in microbiology · 2025Article
- A global deep terrestrial biosphere core microbiome.ISME communications · 2025Article
- Spatial and temporal groundwater biogeochemical variability help inform subsurface connectivity within a high-altitude Alpine catchment (Riale di Ronco, Switzerland).Frontiers in microbiology · 2025Article
- Microbial ecology of the deep terrestrial subsurface.The ISME journal · 2024Review
- Novel candidate taxa contribute to key metabolic processes in Fennoscandian Shield deep groundwaters.ISME communications · 2024Article
- Substrate Specificity of Biofilms Proximate to Historic Shipwrecks.Microorganisms · 2023Article
- Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield.NPJ biofilms and microbiomes · 2023Article
- Transcriptome Analysis of Cyclooctasulfur Oxidation and Reduction by the Neutrophilic ChemolithoautotrophicAntioxidants (Basel, Switzerland) · 2023Article
- Connectivity of Fennoscandian Shield terrestrial deep biosphere microbiomes with surface communities.Communications biology · 2022Article
- Epilithic Microbial Community Functionality in Deep Oligotrophic Continental Bedrock.Frontiers in microbiology · 2022Article
- Strategies to prevent, curb and eliminate biofilm formation based on the characteristics of various periods in one biofilm life cycle.Frontiers in cellular and infection microbiology · 2022Review
- The Fennoscandian Shield deep terrestrial virosphere suggests slow motion 'boom and burst' cycles.Communications biology · 2021Article
- Effects ofFrontiers in microbiology · 2021Article
- Microbial Diversity Under the Influence of Natural Gas Storage in a Deep Aquifer.Frontiers in microbiology · 2021Article
- Rock Surface Fungi in Deep Continental Biosphere-Exploration of Microbial Community Formation with Subsurface In Situ Biofilm Trap.Microorganisms · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDeep terrestrial biosphere waters are separated from the light-driven surface by the time required to percolate to the subsurface. Despite biofilms being the dominant form of microbial life in many natural environments, they have received little attention in the oligotrophic and anaerobic waters found in deep bedrock fractures. This study is the first to use community DNA sequencing to describe biofilm formation under in situ conditions in the deep terrestrial biosphere.
resultsIn this study, flow cells were attached to boreholes containing either "modern marine" or "old saline" waters of different origin and degree of isolation from the light-driven surface of the earth. Using 16S rRNA gene sequencing, we showed that planktonic and attached populations were dissimilar while gene frequencies in the metagenomes suggested that hydrogen-fed, carbon dioxide- and nitrogen-fixing populations were responsible for biofilm formation across the two aquifers. Metagenome analyses further suggested that only a subset of the populations were able to attach and produce an extracellular polysaccharide matrix. Initial biofilm formation is thus likely to be mediated by a few bacterial populations which were similar to Epsilonproteobacteria, Deltaproteobacteria, Betaproteobacteria, Verrucomicrobia, and unclassified bacteria.
conclusionsPopulations potentially capable of attaching to a surface and to produce extracellular polysaccharide matrix for attachment were identified in the terrestrial deep biosphere. Our results suggest that the biofilm populations were taxonomically distinct from the planktonic community and were enriched in populations with a chemolithoautotrophic and diazotrophic metabolism coupling hydrogen oxidation to energy conservation under oligotrophic conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.