ArticleJournal of natural products2017
Prioritizing Natural Product Diversity in a Collection of 146 Bacterial Strains Based on Growth and Extraction Protocols.
Article in Journal of natural products, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
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- Accessing the specialized metabolome of actinobacteria from the bulk soil of Paullinia cupana Mart. on the Brazilian Amazon: a promising source of bioactive compounds against soybean phytopathogens.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Rationally Minimizing Natural Product Libraries Using Mass Spectrometry.bioRxiv : the preprint server for biology · 2024Article
- Metabolomics-Guided Discovery of New Dimeric Xanthones from Co-Cultures of Mangrove Endophytic FungiMarine drugs · 2024Article
- OSMAC Method to Assess Impact of Culture Parameters on Metabolomic Diversity and Biological Activity of Marine-Derived Actinobacteria.Marine drugs · 2023Article
- Molecular Networking and Bioassay-Guided Preparation and Separation of Active Extract and Constituents fromAntioxidants (Basel, Switzerland) · 2023Article
- Case Studies in Molecular Network-Guided Marine Biodiscovery.Marine drugs · 2023Article
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- Prioritization of Microorganisms Isolated from the Indian Ocean SpongeMicroorganisms · 2023Article
- iPRESTO: Automated discovery of biosynthetic sub-clusters linked to specific natural product substructures.PLoS computational biology · 2023Article
- Enhanced correlation-based linking of biosynthetic gene clusters to their metabolic products through chemical class matching.Microbiome · 2023Article
- Metabologenomics analysis ofFrontiers in microbiology · 2023Article
- Microbial Metabolites Annotation by Mass Spectrometry-Based Metabolomics.Advances in experimental medicine and biology · 2023Article
- MS/MS-Based Molecular Networking: An Efficient Approach for Natural Products Dereplication.Molecules (Basel, Switzerland) · 2022Review
- Good practices and recommendations for using and benchmarking computational metabolomics metabolite annotation tools.Metabolomics : Official journal of the Metabolomic Society · 2022Review
- A Metabolomics-Based Toolbox to Assess and Compare the Metabolic Potential of Unexplored, Difficult-to-Grow Bacteria.Marine drugs · 2022Article
1 more citing papers are in PubMed but not listed here.
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9 authors.
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Abstract
In order to expedite the rapid and efficient discovery and isolation of novel specialized metabolites, while minimizing the waste of resources on rediscovery of known compounds, it is crucial to develop efficient approaches for strain prioritization, rapid dereplication, and the assessment of favored cultivation and extraction conditions. Herein we interrogated bacterial strains by systematically evaluating cultivation and extraction parameters with LC-MS/MS analysis and subsequent dereplication through the Global Natural Product Social Molecular Networking (GNPS) platform. The developed method is fast, requiring minimal time and sample material, and is compatible with high-throughput extract analysis, thereby streamlining strain prioritization and evaluation of culturing parameters. With this approach, we analyzed 146 marine Salinispora and Streptomyces strains that were grown and extracted using multiple different protocols. In total, 603 samples were analyzed, generating approximately 1.8 million mass spectra. We constructed a comprehensive molecular network and identified 15 molecular families of diverse natural products and their analogues. The size and breadth of this network shows statistically supported trends in molecular diversity when comparing growth and extraction conditions. The network provides an extensive survey of the biosynthetic capacity of the strain collection and a method to compare strains based on the variety and novelty of their metabolites. This approach allows us to quickly identify patterns in metabolite production that can be linked to taxonomy, culture conditions, and extraction methods, as well as informing the most valuable growth and extraction conditions.
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