ArticleNucleic acids research2017
Enrichment methods provide a feasible approach to comprehensive and adequately powered investigations of the brain methylome.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Guidelines for pre-analytical conditions for assessing the methylation of circulating cell-free DNA.Clinical epigenetics · 2021Pooled it
- Insights from a methylome-wide association study of antidepressant exposure.Nature communications · 2025Article
- The Impact of Childhood Mental Health and Substance Use on Methylation Aging Into Adulthood.Journal of the American Academy of Child and Adolescent Psychiatry · 2024Article
- Investigating neonatal health risk variables through cell-type specific methylome-wide association studies.Clinical epigenetics · 2024Article
- Cell-type specific methylation changes in the newborn child associated to obstetric pain relief.PloS one · 2024Article
- Article
- Genes implicated by a methylome-wide schizophrenia study in neonatal blood show differential expression in adult brain samples.Molecular psychiatry · 2023Article
- Early adversities accelerate epigenetic aging into adulthood: a 10-year, within-subject analysis.Journal of child psychology and psychiatry, and allied disciplines · 2022Article
- Review
- DNA methylation signatures of childhood trauma predict psychiatric disorders and other adverse outcomes 17 years after exposure.Molecular psychiatry · 2022Article
- Dual methylation and hydroxymethylation study of alcohol use disorder.Addiction biology · 2022Article
- Cell-free DNA methylome profiling by MBD-seq with ultra-low input.Epigenetics · 2022Article
- Computational challenges in detection of cancer using cell-free DNA methylation.Computational and structural biotechnology journal · 2022Review
- Methylomic Investigation of Problematic Adolescent Cannabis Use and Its Negative Mental Health Consequences.Journal of the American Academy of Child and Adolescent Psychiatry · 2021Article
- Cancer Detection and Classification by CpG Island Hypermethylation Signatures in Plasma Cell-Free DNA.Cancers · 2021Article
- DNA methylation of the KLK8 gene in depression symptomatology.Clinical epigenetics · 2021Article
- Decreased DNA methylation at promoters and gene-specific neuronal hypermethylation in the prefrontal cortex of patients with bipolar disorder.Molecular psychiatry · 2021Article
- Integrated Transcriptomic and Epigenetic Study of PCOS: Impact ofFrontiers in genetics · 2021Article
- Methylome-wide association findings for major depressive disorder overlap in blood and brain and replicate in independent brain samples.Molecular psychiatry · 2020Article
- A methylation study of long-term depression risk.Molecular psychiatry · 2020Article
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9 authors.
Funding
Abstract
Methylome-wide association studies are typically performed using microarray technologies that only assay a very small fraction of the CG methylome and entirely miss two forms of methylation that are common in brain and likely of particular relevance for neuroscience and psychiatric disorders. The alternative is to use whole genome bisulfite (WGB) sequencing but this approach is not yet practically feasible with sample sizes required for adequate statistical power. We argue for revisiting methylation enrichment methods that, provided optimal protocols are used, enable comprehensive, adequately powered and cost-effective genome-wide investigations of the brain methylome. To support our claim we use data showing that enrichment methods approximate the sensitivity obtained with WGB methods and with slightly better specificity. However, this performance is achieved at <5% of the reagent costs. Furthermore, because many more samples can be sequenced simultaneously, projects can be completed about 15 times faster. Currently the only viable option available for comprehensive brain methylome studies, enrichment methods may be critical for moving the field forward.
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