ArticleNucleic acids research2017
Random pseuoduridylation in vivo reveals critical region of Escherichia coli 23S rRNA for ribosome assembly.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- The mutation of pseudouridine synthase gene BrTRUD1 resulted in glossy leaf in Chinese cabbage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Article
- Metabolism of Epigenetic Ribonucleosides Leads to Nucleolar Stress and Cytotoxicity.ACS chemical biology · 2026Article
- Functions and therapeutic applications of pseudouridylation.Nature reviews. Molecular cell biology · 2025Review
- Adaptive Evolution ofMicroorganisms · 2025Article
- Ribosomal RNA modification enzymes stimulate large ribosome subunit assembly in E. coli.Nucleic acids research · 2024Article
- Late consolidation of rRNA structure during co-transcriptional assembly inbioRxiv : the preprint server for biology · 2024Article
- Aging-induced pseudouridine synthase 10 impairs hematopoietic stem cells.Haematologica · 2023Article
- Article
- RNA modifications: importance in immune cell biology and related diseases.Signal transduction and targeted therapy · 2022Review
- CryoEM structures of pseudouridine-free ribosome suggest impacts of chemical modifications on ribosome conformations.Structure (London, England : 1993) · 2022Article
- SnoRNA guide activities: real and ambiguous.RNA (New York, N.Y.) · 2021Article
- Pseudouridine Synthase RsuA Captures an Assembly Intermediate that Is Stabilized by Ribosomal Protein S17.Biomolecules · 2020Article
- Pseudouridine-Free Escherichia coli Ribosomes.Journal of bacteriology · 2018Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudouridine is the most common modified nucleoside in RNA, which is found in stable RNA species and in eukaryotic mRNAs. Functional analysis of pseudouridine is complicated by marginal effect of its absence. We demonstrate that excessive pseudouridines in rRNA inhibit ribosome assembly. Ten-fold increase of pseudouridines in the 16S and 23S rRNA made by a chimeric pseudouridine synthase leads to accumulation of the incompletely assembled large ribosome subunits. Hyper modified 23S rRNA is found in the r-protein assembly defective particles and are selected against in the 70S and polysome fractions showing modification interference. Eighteen positions of 23S rRNA were identified where isomerization of uridines interferes with ribosome assembly. Most of the interference sites are located in the conserved core of the large subunit, in the domain 0 of 23S rRNA, around the peptide exit tunnel. A plausible reason for pseudouridine-dependent inhibition of ribosome assembly is stabilization of rRNA structure, which leads to the folding traps of rRNA and to the retardation of the ribosome assembly.
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