Evidence map›Paper›PMID 28334681›Full record

ArticleRedox biology2017

Fluorescent proteins such as eGFP lead to catalytic oxidative stress in cells.

Douglas Ganini, Fabian Leinisch, Ashutosh Kumar, JinJie Jiang, Erik J Tokar, Christine C Malone, Robert M Petrovich, Ronald P Mason

Open access · goldAbstract read
In one paragraph

Article in Redox biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.

0numbers the graph read from it
0cells of the map it votes in
73citing papers in PubMed
17.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

73 citing papers in PubMed, 125 citations in OpenAlex.

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  18. Expanding the genetic toolbox forApplied and environmental microbiology · 2024
    Article
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13 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Douglas GaniniFree Radical Biology, Immunity, Inflammation & Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
Fabian LeinischFree Radical Biology, Immunity, Inflammation & Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
Ashutosh KumarFree Radical Biology, Immunity, Inflammation & Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
JinJie JiangFree Radical Biology, Immunity, Inflammation & Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
Erik J TokarStem Cell Toxicology Group, National Toxicology Program Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
Christine C MaloneProtein Expression Core Facility, Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
Robert M PetrovichProtein Expression Core Facility, Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
Ronald P MasonFree Radical Biology, Immunity, Inflammation & Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA. Electronic address: mason4@niehs.nih.gov.
National Institute of Environmental Health Sciences · USNational Institutes of Health · USProtein Express (United States) · US

Funding

Gene Expression and MicroarraysZICES102445 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI GERRISH, KEVIN · 2009 to 2025
$31.6M
Stem Cell ToxicologyZIAES102925 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI TOKAR, ERIK · 2010 to 2021
$25.3M
Protein Expression FacilityZICES102487 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI PETROVICH, ROBERT · 2009 to 2025
$23.9M
Protein Derived RadicalsZIAES050139 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI MASON, RONALD P · 2009 to 2018
$17.5M
In Vivo Detection of Free Radical GenerationZIAES050117 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI MASON, RONALD P · 2009 to 2018
$6.9M
Biomarkers Of Oxidative Stress StudyZIAES048012 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI MASON, RONALD P · 2009 to 2018
$3.0M
6 · The paper itself

Abstract

Fluorescent proteins are an important tool that has become omnipresent in life sciences research. They are frequently used for localization of proteins and monitoring of cells [1,2]. Green fluorescent protein (GFP) was the first and has been the most used fluorescent protein. Enhanced GFP (eGFP) was optimized from wild-type GFP for increased fluorescence yield and improved expression in mammalian systems [3]. Many GFP-like fluorescent proteins have been discovered, optimized or created, such as the red fluorescent protein TagRFP [4]. Fluorescent proteins are expressed colorless and immature and, for eGFP, the conversion to the fluorescent form, mature, is known to produce one equivalent of hydrogen peroxide (H

Indexed as

CatalysisGreen Fluorescent ProteinsHEK293 CellsHeLa CellsHumansHydrogen PeroxideHypoxia-Inducible Factor 1, alpha SubunitLuminescent ProteinsNADOxidative StressRed Fluorescent ProteinSuperoxidesenhanced green fluorescent proteinGreen Fluorescent ProteinsHIF1A protein, humanHydrogen PeroxideHypoxia-Inducible Factor 1, alpha SubunitLuminescent ProteinsNADRed Fluorescent ProteinSuperoxidesFree radicalsGFPH(2)O(2) (hydrogen peroxide)O(2)(•–) (superoxide free radical anion)Oxidative stressRedox biology

Identifiers

PMID28334681
PMCPMC5362137
OpenAlexW2591605089

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.