Evidence map›Paper›PMID 28333993›Full record

ArticlePloS one2017

Integrated transcriptomic and proteomic analysis of the molecular cargo of extracellular vesicles derived from porcine adipose tissue-derived mesenchymal stem cells.

Alfonso Eirin, Xiang-Yang Zhu, Amrutesh S Puranik, John R Woollard, Hui Tang, Surendra Dasari, Amir Lerman, Andre J van Wijnen, Lilach O Lerman

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.

0numbers the graph read from it
0cells of the map it votes in
76citing papers in PubMed
6.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

76 citing papers in PubMed, 100 citations in OpenAlex.

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16 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Alfonso EirinDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, United States of America.
Xiang-Yang ZhuDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, United States of America.
Amrutesh S PuranikDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, United States of America.
John R WoollardDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, United States of America.
Hui TangDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, United States of America.
Surendra DasariDepartment of Health Sciences Research, Mayo Clinic, Rochester, Minnesota, United States of America.
Amir LermanDepartment of Cardiovascular Diseases, Mayo Clinic, Rochester, Minnesota, United States of America.
Andre J van WijnenDepartment of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, United States of America.
Lilach O LermanDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, United States of America.ORCID http://orcid.org/0000-0002-3271-3887
Mayo Clinic · USMayo Clinic in Florida · US

Funding

A potential role for mitoprotection in preserving the kidney in metabolic syndrome and renal artery stenosisK08DK106427 · NIDDK · MAYO CLINIC ROCHESTER · PI EIRIN, ALFONSO · 2015 to 2019
$819k
NIDDK NIH HHS K08 DK106427
6 · The paper itself

Abstract

backgroundMesenchymal stromal/stem cell (MSC) transplantation is a promising therapy for tissue regeneration. Extracellular vesicles (EVs) released by MSCs act as their paracrine effectors by delivering proteins and genetic material to recipient cells. To assess how their cargo mediates biological processes that drive their therapeutic effects, we integrated miRNA, mRNA, and protein expression data of EVs from porcine adipose tissue-derived MSCs.

methodsSimultaneous expression profiles of miRNAs, mRNAs, and proteins were obtained by high-throughput sequencing and LC-MS/MS proteomic analysis in porcine MSCs and their daughter EVs (n = 3 each). TargetScan and ComiR were used to predict miRNA target genes. Functional annotation analysis was performed using DAVID 6.7 database to rank primary gene ontology categories for the enriched mRNAs, miRNA target genes, and proteins. STRING was used to predict associations between mRNA and miRNA target genes.

resultsDifferential expression analysis revealed 4 miRNAs, 255 mRNAs, and 277 proteins enriched in EVs versus MSCs (fold change >2, p<0.05). EV-enriched miRNAs target transcription factors (TFs) and EV-enriched mRNAs encode TFs, but TF proteins are not enriched in EVs. Rather, EVs are enriched for proteins that support extracellular matrix remodeling, blood coagulation, inflammation, and angiogenesis.

conclusionsPorcine MSC-derived EVs contain a genetic cargo of miRNAs and mRNAs that collectively control TF activity in EVs and recipient cells, as well as proteins capable of modulating cellular pathways linked to tissue repair. These properties provide the fundamental basis for considering therapeutic use of EVs in tissue regeneration.

Indexed as

Adipose TissueAnimalsExtracellular VesiclesGene Expression ProfilingMesenchymal Stem CellsMicroRNAsProteinsProteomicsRNA, MessengerSwineMicroRNAsProteinsRNA, Messenger

Identifiers

PMID28333993
PMCPMC5363917
OpenAlexW2602022708

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.