ArticleMolecular biology and evolution2017
Single Cell Transcriptomics, Mega-Phylogeny, and the Genetic Basis of Morphological Innovations in Rhizaria.
Article in Molecular biology and evolution, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 66 citations in OpenAlex.
- Unravelling Evolutionary and Ecological Insights of Foraminifera by Using Next Generation Sequencing: A Review.Biochemical genetics · 2026Review
- Unravelling Evolutionary and Ecological Insights of Foraminifera by Using Next Generation Sequencing: A Review.Biochemical genetics · 2026Review
- Lost in translation: conserved amino acid usage despite extreme codon bias in foraminifera.mBio · 2025Article
- Complex evolutionary patterns within the tubulin gene family of ciliates, unicellular eukaryotes with diverse microtubular structures.BMC biology · 2024Article
- Comparative genomics of Ascetosporea gives new insight into the evolutionary basis for animal parasitism in Rhizaria.BMC biology · 2024Article
- Towards high-throughput parallel imaging and single-cell transcriptomics of microbial eukaryotic plankton.PloS one · 2024Article
- Article
- Gene Duplications Trace Mitochondria to the Onset of Eukaryote Complexity.Genome biology and evolution · 2021Article
- A single-cell genome perspective on studying intracellular associations in unicellular eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Article
- Rhizarian 'Novel Clade 10' Revealed as Abundant and Diverse Planktonic and Terrestrial Flagellates, including Aquavolon n. gen.The Journal of eukaryotic microbiology · 2018Article
- Multigene phylogeny and cell evolution of chromist infrakingdom Rhizaria: contrasting cell organisation of sister phyla Cercozoa and Retaria.Protoplasma · 2018Article
- The immunophilin repertoire of Plasmodiophora brassicae and functional analysis of PbCYP3 cyclophilin.Molecular genetics and genomics : MGG · 2018Article
- Alpha-actinin of the chlorarchiniophytePeerJ · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The innovation of the eukaryote cytoskeleton enabled phagocytosis, intracellular transport, and cytokinesis, and is largely responsible for the diversity of morphologies among eukaryotes. Still, the relationship between phenotypic innovations in the cytoskeleton and their underlying genotype is poorly understood. To explore the genetic mechanism of morphological evolution of the eukaryotic cytoskeleton, we provide the first single cell transcriptomes from uncultured, free-living unicellular eukaryotes: the polycystine radiolarian Lithomelissa setosa (Nassellaria) and Sticholonche zanclea (Taxopodida). A phylogenomic approach using 255 genes finds Radiolaria and Foraminifera as separate monophyletic groups (together as Retaria), while Cercozoa is shown to be paraphyletic where Endomyxa is sister to Retaria. Analysis of the genetic components of the cytoskeleton and mapping of the evolution of these on the revised phylogeny of Rhizaria reveal lineage-specific gene duplications and neofunctionalization of α and β tubulin in Retaria, actin in Retaria and Endomyxa, and Arp2/3 complex genes in Chlorarachniophyta. We show how genetic innovations have shaped cytoskeletal structures in Rhizaria, and how single cell transcriptomics can be applied for resolving deep phylogenies and studying gene evolution in uncultured protist species.
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