ArticleScientific reports2017
Effect of probe diffusion on the SOFI imaging accuracy.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Cross-Correlation Increases Sampling in Diffusion-Based Super-Resolution Optical Fluctuation Imaging.Chemical & biomedical imaging · 2024Article
- Design of experiments for the optimization of SOFI super-resolution microscopy imaging.Biomedical optics express · 2021Article
- Smoothness correction for better SOFI imaging.Scientific reports · 2021Article
- Spectral cross-cumulants for multicolor super-resolved SOFI imaging.Nature communications · 2020Article
- Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface.Cell chemical biology · 2018Article
- Observing the Assembly of Protein Complexes in Living Eukaryotic Cells in Super-Resolution Using refSOFI.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Correcting for photodestruction in super-resolution optical fluctuation imaging.Scientific reports · 2017Article
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Authors and funding
2 authors.
Funding
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Abstract
Live-cell super-resolution fluorescence imaging is becoming commonplace for exploring biological systems, though sample dynamics can affect the imaging quality. In this work we evaluate the effect of probe diffusion on super-resolution optical fluctuation imaging (SOFI), using a theoretical model and numerical simulations based on the imaging of live cells labelled with photochromic fluorescent proteins. We find that, over a range of physiological conditions, fluorophore diffusion results in a change in the amplitude of the SOFI signal. The magnitude of this change is approximately proportional to the on-time ratio of the fluorophores. However, for photochromic fluorescent proteins this effect is unlikely to present a significant distortion in practical experiments in biological systems. Due to this lack of distortions, probe diffusion strongly enhances the SOFI imaging by avoiding spatial undersampling caused by the limited labeling density.
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