ArticleScientific reports2017
Integrated DNA and RNA extraction using magnetic beads from viral pathogens causing acute respiratory infections.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 98 citations in OpenAlex.
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- Investigating the prevalence of three medically important pathogens inFrontiers in parasitology · 2025Article
- Comprehensive Review on Viral RNA Extraction Strategies for Enhanced Molecular Diagnostics.Interdisciplinary perspectives on infectious diseases · 2025Review
- The surface modification of the silica-coated magnetic nanoparticles and their application in molecular diagnostics of virus infection.Scientific reports · 2024Article
- Palm-Sized Lab-In-A-Magnetofluidic Tube Platform for Rapid and Sensitive Virus Detection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Silica-coated magnetic particles for efficient RNA extraction for SARS-CoV-2 detection.Heliyon · 2024Article
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- A semi-automated and high-throughput approach for the detection of honey bee viruses in bee samples.PloS one · 2024Article
- CRISPR Assays for Disease Diagnosis: Progress to and Barriers Remaining for Clinical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium.Molecules (Basel, Switzerland) · 2023Article
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- Development and application of LAMP assays for the detection of enteric adenoviruses in feces.Microbiology spectrum · 2022Article
- Rapid Nucleic Acid Extraction for Aquatic Animal DNA Virus Determination Using Chelex 100 Resin via Conventional PCR and Digital Droplet PCR Detection.Animals : an open access journal from MDPI · 2022Article
- A comparison of SARS-CoV-2 RNA extraction with the QuickGene-810 Nucleic Acid Isolation System compared to the EZ1 Advanced DSP Virus Kit.Access microbiology · 2022Article
- Advancements in Testing Strategies for COVID-19.Biosensors · 2022Review
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- Photo-Cross-Linked Probe-Modified Magnetic Particles for the Selective and Reliable Recovery of Nucleic Acids.ACS omega · 2022Article
- Filtration-assisted magnetofluidic cartridge platform for HIV RNA detection from blood.Lab on a chip · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current extraction methods often extract DNA and RNA separately, and few methods are capable of co-extracting DNA and RNA from sputum. We established a nucleic acid co-extraction method from sputum based on magnetic beads and optimized the method by evaluating influencing factors, such as the guanidinium thiocyanate (GTC) and dithiothreitol (DTT) concentrations, magnetic bead amount, incubation temperature, lysis buffer pH and RNA carrier type. The feasibility of the simultaneous nucleic acid co-extraction method was evaluated by amplifying DNA and RNA viruses from a single clinical specimen with a multiplex RT-qPCR method. Both DNA and RNA were most efficiently extracted when the GTC and DTT concentrations were 2.0 M and 80 mM, respectively, 20 μl magnetic beads were added, the incubation temperature was 80 °C, the pH was 8 or 9, and RNA carrier A was used. Therefore, we established a simple method to extract nucleic acids from two important respiratory viruses compared with other commercial kits. This magnetic beads-based co-extraction method for sputum followed by a multiplex RT-qPCR can rapidly and precisely detect DNA and RNA viruses from a single clinical specimen and has many advantages, such as decreased time, low cost, and a lack of harmful chemicals.
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Registered trials
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