ArticleNature communications2017
Cell-free reconstitution reveals centriole cartwheel assembly mechanisms.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 90 citations in OpenAlex.
- Towards a systems-level view of the microtubule cytoskeleton and its functions in physiology and disease.Nature reviews. Molecular cell biology · 2026Review
- Critical constituents and assembly principles of centriole biogenesis in human cells.Nature reviews. Molecular cell biology · 2026Review
- Visualizing Drosophila centrioles by expansion microscopy.Journal of cell science · 2026Article
- The luminal ring protein C2CD3 acts as a radial in-to-out organizer of the distal centriole and appendages.PLoS biology · 2025Article
- Poc1 bridges basal body inner junctions to promote triplet microtubule integrity and connections.The Journal of cell biology · 2024Article
- The kinase ZYG-1 phosphorylates the cartwheel protein SAS-5 to drive centriole assembly in C. elegans.EMBO reports · 2024Article
- Article
- Trafficking in cancer: from gene deregulation to altered organelles and emerging biophysical properties.Frontiers in cell and developmental biology · 2024Review
- Poc1 is a basal body inner junction protein that promotes triplet microtubule integrity and interconnections.bioRxiv : the preprint server for biology · 2023Article
- A biallelic frameshift indel in PPP1R35 as a cause of primary microcephaly.American journal of medical genetics. Part A · 2023Article
- Bld10p/Cep135 determines the number of triplets in the centriole independently of the cartwheel.The EMBO journal · 2022Article
- Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel.Structure (London, England : 1993) · 2022Article
- Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture.Nature communications · 2021Article
- Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly.Nature communications · 2021Article
- A first-takes-all model of centriole copy number control based on cartwheel elongation.PLoS computational biology · 2021Article
- Superresolution characterization of core centriole architecture.The Journal of cell biology · 2021Article
- Primary cilia biogenesis and associated retinal ciliopathies.Seminars in cell & developmental biology · 2021Review
- Article
- Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography.The EMBO journal · 2020Article
- Architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography.The EMBO journal · 2020Article
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How cellular organelles assemble is a fundamental question in biology. The centriole organelle organizes around a nine-fold symmetrical cartwheel structure typically ∼100 nm high comprising a stack of rings that each accommodates nine homodimers of SAS-6 proteins. Whether nine-fold symmetrical ring-like assemblies of SAS-6 proteins harbour more peripheral cartwheel elements is unclear. Furthermore, the mechanisms governing ring stacking are not known. Here we develop a cell-free reconstitution system for core cartwheel assembly. Using cryo-electron tomography, we uncover that the Chlamydomonas reinhardtii proteins CrSAS-6 and Bld10p together drive assembly of the core cartwheel. Moreover, we discover that CrSAS-6 possesses autonomous properties that ensure self-organized ring stacking. Mathematical fitting of reconstituted cartwheel height distribution suggests a mechanism whereby preferential addition of pairs of SAS-6 rings governs cartwheel growth. In conclusion, we have developed a cell-free reconstitution system that reveals fundamental assembly principles at the root of centriole biogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.