Evidence map›Paper›PMID 28332161›Full record

ArticleCurrent microbiology2017

Real-Time PCR Identification of Six Malassezia Species.

Amin Ilahi, Inès Hadrich, Sourour Neji, Houaida Trabelsi, Fattouma Makni, Ali Ayadi

Abstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 33 citations in OpenAlex.

  1. Trial
  2. First case of neonatal bloodstream infection caused byExperimental and therapeutic medicine · 2026
    Article
  3. Article
  4. Journal of fungi (Basel, Switzerland) · 2025
    Review
  5. Prevalence ofOpen veterinary journal · 2025
    Article
  6. Direct molecular analysis ofCurrent medical mycology · 2023
    Article
  7. Genotypic Analysis of the Population Structure inJournal of fungi (Basel, Switzerland) · 2023
    Article
  8. Production and Quantification of Virulence Factors inPolish journal of microbiology · 2022
    Article
  9. Journal of fungi (Basel, Switzerland) · 2022
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Quantification of Malassezia pachydermatis by real-time PCR in swabs from the external ear canal of dogs.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Amin IlahiFungal and Parasitic Molecular Biology Laboratory, Faculty of Medicine, University of Sfax, Rue Magida Boulila, Sfax, 3029, Tunisia.
Inès HadrichFungal and Parasitic Molecular Biology Laboratory, Faculty of Medicine, University of Sfax, Rue Magida Boulila, Sfax, 3029, Tunisia. ineshadrich@yahoo.fr.
Sourour NejiFungal and Parasitic Molecular Biology Laboratory, Faculty of Medicine, University of Sfax, Rue Magida Boulila, Sfax, 3029, Tunisia.
Houaida TrabelsiFungal and Parasitic Molecular Biology Laboratory, Faculty of Medicine, University of Sfax, Rue Magida Boulila, Sfax, 3029, Tunisia.
Fattouma MakniFungal and Parasitic Molecular Biology Laboratory, Faculty of Medicine, University of Sfax, Rue Magida Boulila, Sfax, 3029, Tunisia.
Ali AyadiFungal and Parasitic Molecular Biology Laboratory, Faculty of Medicine, University of Sfax, Rue Magida Boulila, Sfax, 3029, Tunisia.
University of Sfax · TN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lipophilic yeast Malassezia species is widely found on the skin surface of humans and other animals. This fungus can cause pityriasis versicolor, Malassezia folliculitis, and seborrheic dermatitis. Still now, there is a problem with species identification of Malassezia with conventional methods. We developed a real-time polymerase chain reaction (PCR) assay with multiple hybridization probes for detecting M. globosa, M. furfur, M. restricta, M. sympodialis, M. slooffiae, and M. pachydermatis. The amplification curves and specific melting peaks of the probes hybridized with real-time PCR product were used for species identifications. The assay was further evaluated on 120 samples which were performed by swabbing from 60 domestic animals (23 goats, 10 dogs, 15 cows, 3 cats, 8 rabbits, and 1 donkey) and in 70 human samples (28 patients with pityriasis versicolor, 17 breeders, and 25 control group). Fifteen M. pachydermatis were identified from animals. From human, 61 isolates were identified as M. globosa (28), M. furfur (15), M. restricta (6), M. sympodialis (8), M. slooffiae (2), and M. pachydermatis (2). Eight cases of co-detection from 6 patients and 2 breeders were revealed. Our findings show that the assay was highly effective in identifying Malassezia species. The application of multiplex real-time PCR provides a sensitive and rapid identification system for Malassezia species, which may be applied in further epidemiological surveys from clinical samples.

Indexed as

AnimalsCatsCattleDogsEquidaeGoatsHumansMalasseziaMolecular TypingMycological Typing TechniquesNucleic Acid DenaturationRabbitsReal-Time Polymerase Chain ReactionRNA, RibosomalSkinTinea VersicolorRNA, RibosomalRNA, ribosomal, 26SDomestic animalsMalasseziaMolecular identificationPCRProbes

Identifiers

PMID28332161
OpenAlexW2602980255

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.