ArticleScientific reports2017
Efficient Generation of diRNAs Requires Components in the Posttranscriptional Gene Silencing Pathway.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- SSA-mediated selection marker gene activation enhances relative gene targeting efficiency in plants.Horticulture research · 2025Article
- Double step screening using endogenous marker improves relative gene targeting efficiency in Arabidopsis.Scientific reports · 2024Article
- Chromatin dynamics and RNA metabolism are double-edged swords for the maintenance of plant genome integrity.Nature plants · 2024Review
- The plant siRNA landscape.The Plant cell · 2024Review
- Precise and heritable gene targeting in rice using a sequential transformation strategy.Cell reports methods · 2023Article
- Pol V produced RNA facilitates transposable element excision site repair inmicroPublication biology · 2023Article
- Plant gene silencing signals move from the phloem to influence gene expression in shoot apical meristems.BMC plant biology · 2022Article
- Human rDNA and Cancer.Cells · 2021Review
- Gene Targeting Facilitated by Engineered Sequence-Specific Nucleases: Potential Applications for Crop Improvement.Plant & cell physiology · 2021Review
- Article
- New Faces of old Friends: Emerging new Roles of RNA-Binding Proteins in the DNA Double-Strand Break Response.Frontiers in molecular biosciences · 2021Review
- RNA: a double-edged sword in genome maintenance.Nature reviews. Genetics · 2020Review
- The Role of Small Noncoding RNA in DNA Double-Strand Break Repair.International journal of molecular sciences · 2020Review
- Conserved chromosomal functions of RNA interference.Nature reviews. Genetics · 2020Review
- The roles of RNA in DNA double-strand break repair.British journal of cancer · 2020Review
- The Role of Noncoding RNAs in Double-Strand Break Repair.Frontiers in plant science · 2019Review
- Next-generation sequencing reveals two populations of damage-induced small RNAs at endogenous DNA double-strand breaks.Nucleic acids research · 2018Article
- Manipulating plant RNA-silencing pathways to improve the gene editing efficiency of CRISPR/Cas9 systems.Genome biology · 2018Article
- CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation.Nature communications · 2018Article
- From "Cellular" RNA to "Smart" RNA: Multiple Roles of RNA in Genome Stability and Beyond.Chemical reviews · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
Abstract
It has been reported that double-stranded break (DSB)-induced small RNAs (diRNAs) are generated via the RNA-directed DNA methylation pathway and function in DSB repair in Arabidposis. However, important questions remain regarding the biogenesis and function of diRNAs. Here, we used CRISPR/Cas9- or TALEN-triggered DSBs to characterize diRNAs in Arabidopsis and rice. We found that 21-nt diRNAs were generated from a 35S promoter::GU-US reporter transgene targeted by CRISPR/Cas9. Unexpectedly, Pol II transcription of the transgene was required for efficient diRNA production and the level of diRNA accumulation correlated with the expression level of the transgene. diRNAs were not detected from CRISPR/Cas9- or TALEN-induced DSBs within the examined endogenous genes in Arabidopsis or rice. We also found that DCL4 and RDR6 that are known to be involved in posttranscriptional gene silencing were required to generate diRNAs. Our results suggest that DSBs are necessary but not sufficient for efficient diRNA generation and a high level of diRNAs is not necessary for DSB repair.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.