Evidence map›Paper›PMID 28330602›Full record

ArticleEBioMedicine2017

The Pneumococcal Alpha-Glycerophosphate Oxidase Enhances Nasopharyngeal Colonization through Binding to Host Glycoconjugates.

Layla K Mahdi, Melanie A Higgins, Christopher J Day, Joe Tiralongo, Lauren E Hartley-Tassell, Michael P Jennings, David L Gordon, Adrienne W Paton, James C Paton, Abiodun D Ogunniyi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EBioMedicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Panel 8: Vaccines and immunology.International journal of pediatric otorhinolaryngology · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Layla K MahdiResearch Centre for Infectious Diseases, Department of Molecular and Cellular Biology, School of Biological Sciences, The University of Adelaide, SA 5005, Australia.
Melanie A HigginsResearch Centre for Infectious Diseases, Department of Molecular and Cellular Biology, School of Biological Sciences, The University of Adelaide, SA 5005, Australia.
Christopher J DayInstitute For Glycomics, Griffith University, Gold Coast, QLD, 4222, Australia.
Joe TiralongoInstitute For Glycomics, Griffith University, Gold Coast, QLD, 4222, Australia.
Lauren E Hartley-TassellInstitute For Glycomics, Griffith University, Gold Coast, QLD, 4222, Australia.
Michael P JenningsInstitute For Glycomics, Griffith University, Gold Coast, QLD, 4222, Australia.
David L GordonDepartment of Microbiology and Infectious Diseases, Flinders University, Bedford Park, SA 5042, Australia.
Adrienne W PatonResearch Centre for Infectious Diseases, Department of Molecular and Cellular Biology, School of Biological Sciences, The University of Adelaide, SA 5005, Australia.
James C PatonResearch Centre for Infectious Diseases, Department of Molecular and Cellular Biology, School of Biological Sciences, The University of Adelaide, SA 5005, Australia. Electronic address: james.paton@adelaide.edu.au.
Abiodun D OgunniyiResearch Centre for Infectious Diseases, Department of Molecular and Cellular Biology, School of Biological Sciences, The University of Adelaide, SA 5005, Australia. Electronic address: david.ogunniyi@adelaide.edu.au.
University of Adelaide · AUGriffith University · AUFlinders University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Streptococcus pneumoniae (the pneumococcus) is a major human pathogen, causing a broad spectrum of diseases including otitis media, pneumonia, bacteraemia and meningitis. Here we examined the role of a potential pneumococcal meningitis vaccine antigen, alpha-glycerophosphate oxidase (SpGlpO), in nasopharyngeal colonization. We found that serotype 4 and serotype 6A strains deficient in SpGlpO have significantly reduced capacity to colonize the nasopharynx of mice, and were significantly defective in adherence to human nasopharyngeal carcinoma cells in vitro. We also demonstrate that intranasal immunization with recombinant SpGlpO significantly protects mice against subsequent nasal colonization by wild type serotype 4 and serotype 6A strains. Furthermore, we show that SpGlpO binds strongly to lacto/neolacto/ganglio host glycan structures containing the GlcNAcβ1-3Galβ disaccharide, suggesting that SpGlpO enhances colonization of the nasopharynx through its binding to host glycoconjugates. We propose that SpGlpO is a promising vaccine candidate against pneumococcal carriage, and warrants inclusion in a multi-component protein vaccine formulation that can provide robust, serotype-independent protection against all forms of pneumococcal disease.

Indexed as

AnimalsBacterial AdhesionBinding SitesCell Line, TumorFemaleGlycerolphosphate DehydrogenaseGlycoconjugatesHumansImmunoglobulin GMiceMolecular Dynamics SimulationNasopharynxPneumococcal VaccinesProtein Structure, TertiaryRecombinant Fusion ProteinsSerogroupGlycerolphosphate DehydrogenaseGlycoconjugatesImmunoglobulin GPneumococcal VaccinesRecombinant Fusion ProteinsAdherenceAlpha-glycerophosphate oxidaseBacterial pathogensColonizationHost glycoconjugatesImmunizationPneumococcal diseaseProtein vaccinesStreptococcus pneumoniae

Identifiers

PMID28330602
PMCPMC5405170
OpenAlexW2594722486

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.