Article3 Biotech2016
A rapid method for isolation of genomic DNA from food-borne fungal pathogens.
Article in 3 Biotech, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
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- Bioprocessing and characterization of thermostable phytase from Aspergillus terreus, an endophyte of Catharanthus roseus, with a potential activity to hydrolyze phytic acid in wheat bran.BMC biotechnology · 2025Article
- Biosourcing and optimization of fungal lipase production from cheap agro waste via solid state fermentation.Scientific reports · 2025Article
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- The Tetracycline-Inducible/CRISPR-Cas9 System is an Efficient Tool for Studying Gene Function in Phytophthora sojae.Molecular plant pathology · 2025Article
- Enzymatic oxidation of galacturonides from pectin breakdown contributes to stealth infection by Oomycota phytopathogens.Nature communications · 2025Article
- Tolerance ofBiofilm · 2024Article
- Antioxidant and Antifungal Properties of Cinnamon, Cloves,Pathogens (Basel, Switzerland) · 2024Article
- PsAF5 functions as an essential adapter for PsPHB2-mediated mitophagy under ROS stress in Phytophthora sojae.Nature communications · 2024Article
- Identification and Characterization ofJournal of fungi (Basel, Switzerland) · 2024Article
- Fungal endophytes from saline-adapted shrubs induce salinity stress tolerance in tomato seedlings.FEMS microbes · 2024Article
- Diversity and Advantages of Culturable Endophytic Fungi from Tea (Journal of fungi (Basel, Switzerland) · 2023Article
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- swnk plays an important role in the biosynthesis of swainsonine in Metarhizium anisopliae.Biotechnology letters · 2023Article
- Occurrence of Heat-Resistant Mold Ascospores in Pineapple and Sugarcane Field Soils in Thailand.International journal of microbiology · 2023Article
- Identification of sources of resistance in cowpea lines to Macrophomina root rot disease in Northern Ghana.Heliyon · 2022Article
- Genome-wide comparison deciphers lifestyle adaptation and glass biodeterioration property of Curvularia eragrostidis C52.Scientific reports · 2022Article
- The Transcription FactorFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Food contaminated with fungal pathogens can cause extremely harmful effects to human even when present in low concentrations. Researchers now pay more attention towards rapid DNA extraction for the quick screening, which is highly demanded in diverse research field. Molecular description of many fungal species is identified by different molecular characteristics. Hence, the efficient isolation of genomic DNA and amplification using PCR is a prerequisite for molecular characterization. Here, we used an improved Sodium dodecyl sulfate-Cetyltrimethyl ammonium bromide-Chloroform-isoamyl alcohol method by combining Sodium dodecyl sulfate with cetyl methylammonium bromide without addition of proteinase K, RNase K, and β-mercaptoethanol. To analyze the quality of recovered DNA, this method was compared with the other four routine methods. The present method has been chosen in the study as a preferred method because of easy adaptation to routine laboratories/food industries considering its rapid, sensitivit,y and cost effectiveness involved in the method.
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