Article3 Biotech2016
A simple and efficient Agrobacterium tumefaciens-mediated plant transformation of Brassica rapa ssp. pekinensis.
Article in 3 Biotech, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Functional study ofFrontiers in plant science · 2022Article
- L-Cysteine Increases the Transformation Efficiency of Chinese Cabbage (Frontiers in plant science · 2021Article
- Production of ascorbic acid, total protein, callus and root in vitro of non-heading Chinese cabbage by tissue culture.Molecular biology reports · 2020Article
- BrmiR828 TargetsInternational journal of molecular sciences · 2020Article
- A convenient, rapid and efficient method for establishing transgenic lines ofPlant methods · 2020Article
- Investigating the In Vitro Regeneration Potential of Commercial Cultivars ofPlants (Basel, Switzerland) · 2019Article
- Transgenic Wucai (Brassica campestris L.) produced via Agrobacterium-mediated anther transformation in planta.Plant cell reports · 2019Article
- High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes.AMB Express · 2018Article
- Article
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Authors and funding
4 authors.
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Abstract
The present study aims to investigate the numerous factors influencing Agrobacterium tumefaciens-mediated genetic transformation of Chinese cabbage (Brassica rapa ssp. pekinensis). Factors affecting transformation efficiency, such as age of explants, Agrobacterium concentration, and effect of acetosyringone, pre-cultivation, infection and co-cultivation time of Agrobacterium were examined. The pre-cultured hypocotyls from young seedlings prior to exposure to Agrobacterium showed higher shoot regeneration. The plant transformation with the modest A. tumefaciens concentrations (0.8 OD) and the 3 days co-cultivation periods increased transformation efficiency. Plant growth hormones [1-naphthyl acetic acid (NAA) and 6-benzyl amino purine (BAP)] were essential for callus and shoot formation. Root formation was effective in half strength MS medium without supplementation of root-inducing hormones. To maintain selection pressure, plant subculture was carried out every 2 weeks with selective antibiotics. The putative transgenic plants were acclimatized in the greenhouse. Polymerase chain reaction was performed to confirm the integration of T-DNA into the genome of transgenic plants. A transformation efficiency of 15 % was obtained. This protocol allows effective transformation and indirect regeneration of Brassica rapa.
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Registered trials
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