Article3 Biotech2016
An efficient and cost-effective method for DNA extraction from athalassohaline soil using a newly formulated cell extraction buffer.
Article in 3 Biotech, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Characterization of bacterial diversity associated with a Salar de Atacama native plant Nitrophila atacamensis.Environmental microbiome · 2025Article
- Characterizing the microbial metagenome of calcareous stromatolite formations in the San Felipe Creek in the Anza Borrego Desert.Microbiology resource announcements · 2024Article
- SURE gel electrophoresis: A method for improved detection and purification of dilute nucleic acid samples.Analytical biochemistry · 2024Article
- Study of Wetland Soils of the Salar de Atacama with Different Azonal Vegetative Formations Reveals Changes in the Microbiota Associated with Hygrophile Plant Type on the Soil Surface.Microbiology spectrum · 2022Article
- Molecular detection and quantification of theWeed research · 2022Article
- Leave no stone unturned: individually adapted xerotolerant Thaumarchaeota sheltered below the boulders of the Atacama Desert hyperarid core.Microbiome · 2021Article
- A low-cost pipeline for soil microbiome profiling.MicrobiologyOpen · 2020Article
- Robust DNA protocols for tropical soils.Heliyon · 2020Article
- Article
- Optimization of high-yielding protocol for DNA extraction from the forest rhizosphere microbes.3 Biotech · 2017Article
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4 authors.
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Abstract
The present study describes the rapid and efficient indirect lysis method for environmental DNA extraction from athalassohaline soil by newly formulated cell extraction buffer. The available methods are mostly based on direct lysis which leads to DNA shearing and co-extraction of extra cellular DNA that influences the community and functional analysis. Moreover, during extraction of DNA by direct lysis from athalassohaline soil, it was observed that, upon addition of poly ethylene glycol (PEG), isopropanol or absolute ethanol for precipitation of DNA, salt precipitates out and affecting DNA yield significantly. Therefore, indirect lysis method was optimized for extraction of environmental DNA from such soil containing high salts and low microbial biomass (CFU 4.3 × 10
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