Article3 Biotech2016
An efficient protocol for isolation of inhibitor-free nucleic acids even from recalcitrant plants.
Article in 3 Biotech, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 74 citations in OpenAlex.
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- Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors.Frontiers in bioengineering and biotechnology · 2025Article
- Optimized protocol for culturing and extracting DNA from fungal isolates associated with brown spot needle blight in pine trees.PloS one · 2025Article
- Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR.Foods (Basel, Switzerland) · 2024Article
- Preliminary insights of the genetic diversity and invasion pathways ofEcology and evolution · 2024Article
- An innovative optimized protocol for high-quality genomic DNA extraction from recalcitrant Shea tree (Vitellaria paradoxa, C.F. Gaertn) plant and its suitability for downstream applications.Molecular biology reports · 2024Article
- Comparing DNA isolation methods for forest trees: quality, plastic footprint, and time-efficiency.Plant methods · 2023Article
- Can Pharmaceutical Excipients Threaten the Aquatic Environment? A Risk Assessment Based on the MicrotoxMolecules (Basel, Switzerland) · 2023Article
- An Efficient Method to Prepare Barcoded cDNA Libraries from Plant Callus for Long-Read Sequencing.Methods and protocols · 2023Article
- CRISPR/Cas9-Mediated Enrichment Coupled to Nanopore Sequencing Provides a Valuable Tool for the Precise Reconstruction of Large Genomic Target Regions.International journal of molecular sciences · 2023Article
- An improvedBiology methods & protocols · 2023Review
- Combination and improvement of conventional DNA extraction methods in Actinobacteria to obtain high-quantity and high-quality DNA.Iranian journal of microbiology · 2022Article
- A Comprehensive High-Quality DNA and RNA Extraction Protocol for a Range of Cultivars and Tissue Types of the Woody Crop Avocado.Plants (Basel, Switzerland) · 2022Article
- DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A modified approach for high-quality RNA extraction of spore-forming Bacillus subtilis at varied physiological stages.Molecular biology reports · 2021Article
- Ex Vivo Study on the Antioxidant Activity of a Winemaking By-Product Polyphenolic Extract (TaurisoloAntioxidants (Basel, Switzerland) · 2021Article
- FITNESS Acts as a Negative Regulator of Immunity and Influences the Plant Reproductive Output AfterFrontiers in plant science · 2021Article
- Pharmaceutical assessment of polyvinylpyrrolidone (PVP): As excipient from conventional to controlled delivery systems with a spotlight on COVID-19 inhibition.Journal of drug delivery science and technology · 2020Review
- A universal method for high-quality RNA extraction from plant tissues rich in starch, proteins and fiber.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For fast and easy isolation of inhibitor-free genomic DNA even from the toughest plant leaf samples, including those high in polyphenols and polysaccharides, a protocol has been developed. To prevent the solubility of polysaccharides in the DNA extract, high salt concentration (1.4 M) was used in the extraction buffer. Polyvinylpyrrolidone (PVP) was used for the removal of polyphenols as polymerase chain reaction (PCR) inhibitors. Proteins like various enzymes were degraded by proteinase K and removed by centrifugation from plant extracts during the isolation process resulting in pure DNA and RNA ready to use in downstream applications including PCR, quantitative polymerase chain reaction (qPCR), ligation, restriction and sequencing. This protocol yielded a high molecular weight DNA and RNA isolated from leaves and roots of recalcitrant plants which was free from contamination and color. The average yields of total RNA from roots and shoot of Betula and Grape ranged from 285 to 364 ng/µl with A260/A280 between 1.9 and 2.08. The RNA isolated with this protocol was verified to be suitable for PCR, quantitative real-time PCR, semi-quantitative reverse transcription polymerase chain reaction, cDNA synthesis and expression analysis. This protocol shown here is reproducible and can be used for a broad spectrum of plant species which have polyphenols and polysaccharide compounds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.