Evidence map›Paper›PMID 28330097›Full record

Article3 Biotech2016

Immobilization of halophilic Aspergillus awamori EM66 exochitinase on grafted k-carrageenan-alginate beads.

Mona A Esawy, Ghada E A Awad, Walaa A Abdel Wahab, Magdy M M Elnashar, Ahmed El-Diwany, Saadia M H Easa, Fawkia M El-Beih

Open access · hybridAbstract read
In one paragraph

Article in 3 Biotech, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.4field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Mona A EsawyDepartment of Chemistry of Microbial and Natural Products, National Research Centre, Tahrir Street, Dokki, Cairo, Egypt.
Ghada E A AwadDepartment of Chemistry of Microbial and Natural Products, National Research Centre, Tahrir Street, Dokki, Cairo, Egypt. ghadaawad18@yahoo.com.
Walaa A Abdel WahabDepartment of Chemistry of Microbial and Natural Products, National Research Centre, Tahrir Street, Dokki, Cairo, Egypt.
Magdy M M ElnasharCentre of Scientific Excellence-Group of Encapsulation and Nanobiotechnology, Polymers Department, National Research Center, El-Behooth St., Dokki, Cairo, Egypt.
Ahmed El-DiwanyDepartment of Chemistry of Microbial and Natural Products, National Research Centre, Tahrir Street, Dokki, Cairo, Egypt.
Saadia M H EasaDepartment of Microbiology, Faculty of Science, Ain Shams University, Khalifa El-Maamon St., Cairo, Egypt.
Fawkia M El-BeihDepartment of Microbiology, Faculty of Science, Ain Shams University, Khalifa El-Maamon St., Cairo, Egypt.
National Research Centre · EGAin Shams University · EGCurtin University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel extreme halophilic exochitinase enzyme was produced by honey isolate Aspergillus awamori EM66. The enzyme was immobilized successfully on k-carrageenan-alginate gel carrier (CA) with 93 % immobilization yield. The immobilization process significantly improved the enzyme specific activity 2.6-fold compared to the free form. The significant factors influencing the immobilization process such as enzyme protein concentration and loading time were studied. Distinguishable characteristics of optimum pH and temperature, stability at different temperatures and NaCl tolerance for free and immobilized enzyme were studied. The immobilization process improved optimum temperature from 35 to 45 °C. The immobilized enzyme retained 76.70 % of its activity after 2 h at 75 °C compared to complete loss of activity for the free enzyme. The reusability test proved the durability of the CA gel beads for 28 cycles without losing its activity.

Indexed as

Aspergillus awamoriExochitinaseHoney isolateImmobilization

Identifiers

PMID28330097
PMCPMC4711283
OpenAlexW2236255397

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.