Evidence map›Paper›PMID 28330092›Full record

Article3 Biotech2016

Development of molecular method for sex identification in date palm (Phoenix dactylifera L.) plantlets using novel sex-linked microsatellite markers.

Maryam, Muhammad Jafar Jaskani, Faisal Saeed Awan, Saeed Ahmad, Iqrar A Khan

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Article in 3 Biotech, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
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5citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

MaryamInstitute of Horticultural Sciences, University of Agriculture Faisalabad, Faisalabad, 38000, Pakistan.
Muhammad Jafar JaskaniInstitute of Horticultural Sciences, University of Agriculture Faisalabad, Faisalabad, 38000, Pakistan. jjaskani@uaf.edu.pk.
Faisal Saeed AwanCentre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, 38000, Pakistan.
Saeed AhmadInstitute of Horticultural Sciences, University of Agriculture Faisalabad, Faisalabad, 38000, Pakistan.
Iqrar A KhanInstitute of Horticultural Sciences, University of Agriculture Faisalabad, Faisalabad, 38000, Pakistan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microsatellite markers containing simple sequence repeats (SSRs) are a valuable tool for genetic analysis. Date palm is a dioecious and slow flowering and is very difficult to identify the gender of the trees until it reaches the reproductive age (5-10 years). A total of 12 microsatellite primers were used with 30 date palm samples, 14 parents (8 male + 6 females) and 16 progeny (developed from parents breeding) which showed that microsatellites were highly polymorphic, having a great number of alleles. A total of 124 alleles were characterized in 12 SSR loci. On average, there are 9.08 alleles per locus, with a range from 5 to 16 alleles, for primers mpdCIR15 and mpdCIR57, respectively. These primers produced 15 polymorphic loci specifically in male date palm samples and the seedlings harboring the unique fragments were further characterized as male plants. Increasingly, 38.46 % of these loci were scored as homozygous alleles while 61.53 % heterozygous allelic loci were determined. Primer mpdCIR48 produced a specific locus (250/250) in all male samples whereas the same locus was absent in female samples. Similarly, a locus of 300/310 bp reoccurred in 5 date palm male samples using marker DP-168 which indicated that these are the promising candidate marker to detect the sex in date palm seedlings at early stage. The data resulted from combination of 12 primers enabled the 16 seedling samples progeny (developed from parents breeding) of date palm cultivars to divide into two groups i.e., male and female regarding their sex expression comparative to the parents (male + female) using the principle coordinate analysis.

Indexed as

Phoenix dactylifera L.PolymorphismSex identificationSSR marker

Identifiers

PMID28330092
PMCPMC4706835

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.