ArticleNature communications2017
Quantitative volumetric Raman imaging of three dimensional cell cultures.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Confocal Raman Microscopy Quantitatively Reveals Subcellular Metabolic Adaptation Mechanisms of Deep-Sea Mussels to Methane Deprivation.Environmental science & technology · 2026Article
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- Exploring In Vitro Mesenchymal Stem Cell Osteodifferentiation via Vibrational Microspectroscopy: A Review.Stem cell reviews and reports · 2025Review
- Chemical imaging for biological systems: techniques, AI-driven processing, and applications.Journal of materials chemistry. B · 2025Review
- Noninvasive andThe Analyst · 2025Article
- Single-Molecule-Sensitive Three-Dimensional Atomic Heterostructures with Extreme Light-Matter Coupling.Journal of the American Chemical Society · 2025Article
- Adaptive Raman spectral unmixing method based on Voigt peak compensation for quantitative analysis of cellular biochemical components.Biomedical optics express · 2025Article
- Raman Microscopy and Bone.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Unveiling the Molecular Secrets: A Comprehensive Review of Raman Spectroscopy in Biological Research.ACS omega · 2024Review
- Article
- Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging.Science advances · 2024Article
- Hyperspectral unmixing for Raman spectroscopy via physics-constrained autoencoders.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Tunable Hybrid Hydrogels of Alginate and Cell-Derived dECM to Study the Impact of Matrix Alterations on Epithelial-to-Mesenchymal Transition.Advanced healthcare materials · 2024Article
- Label-free 3D molecular imaging of living tissues using Raman spectral projection tomography.Nature communications · 2024Article
- RamanSPy: An Open-Source Python Package for Integrative Raman Spectroscopy Data Analysis.Analytical chemistry · 2024Article
- Raman micro-spectroscopy as a tool to study immunometabolism.Biochemical Society transactions · 2024Review
- Noise learning of instruments for high-contrast, high-resolution and fast hyperspectral microscopy and nanoscopy.Nature communications · 2024Article
- Lactate- and immunomagnetic-purified hiPSC-derived cardiomyocytes generate comparable engineered cardiac tissue constructs.JCI insight · 2024Article
- Multimodal Imaging of Pancreatic Cancer Microenvironment in Response to an Antiglycolytic Drug.Advanced healthcare materials · 2023Article
- Quantitative Raman chemical imaging of intracellular drug-membrane aggregates and small molecule drug precipitates in cytoplasmic organelles.Advanced drug delivery reviews · 2023Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. We demonstrate visualization and quantification of fine details in cell shape, cytoplasm, nucleus, lipid bodies and cytoskeletal structures in 3D with unprecedented biomolecular specificity for vibrational microspectroscopy.
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