ReviewJournal of extracellular vesicles2017
Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components?
Review in Journal of extracellular vesicles, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed.
- MicroRNAs Secreted by the Embryo in Spent Culture Medium Can Regulate mRNAs Involved in Endometrial Receptivity, Embryo Attachment, and Invasion.International journal of molecular sciences · 2025Article
- Extracellular vesicles, RNA sequencing, and bioinformatic analyses: Challenges, solutions, and recommendations.Journal of extracellular vesicles · 2024Review
- Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality.BMC genomics · 2024Article
- Small RNA structural biochemistry in a post-sequencing era.Nature protocols · 2024Review
- Culture conditions greatly impact the levels of vesicular and extravesicular Ago2 and RNA in extracellular vesicle preparations.Journal of extracellular vesicles · 2023Article
- Cell culture-derived extracellular vesicles: Considerations for reporting cell culturing parameters.Journal of extracellular biology · 2023Article
- Extracellular Vesicle Depletion Protocols of Foetal Bovine Serum Influence Umbilical Cord Mesenchymal Stromal Cell Phenotype, Immunomodulation, and Particle Release.International journal of molecular sciences · 2023Article
- Mesenchymal Stem Cell-Derived Extracellular Vesicles: Hype or Hope for Skeletal Muscle Anti-Frailty.International journal of molecular sciences · 2023Review
- Methods for the identification and characterization of extracellular vesicles in cardiovascular studies: from exosomes to microvesicles.Cardiovascular research · 2023Article
- Extracellular vesicles and their cells of origin: Open issues in autoimmune diseases.Frontiers in immunology · 2023Review
- Exomeres and Supermeres: monolithic or diverse?Journal of extracellular biology · 2022Article
- Growth Media Conditions Influence the Secretion Route and Release Levels of Engineered Extracellular Vesicles.Advanced healthcare materials · 2022Article
- Distinct small non-coding RNA landscape in the axons and released extracellular vesicles of developing primary cortical neurons and the axoplasm of adult nerves.RNA biology · 2021Article
- Examining the evidence for extracellular RNA function in mammals.Nature reviews. Genetics · 2021Review
- Profiling of Extracellular Small RNAs Highlights a Strong Bias towards Non-Vesicular Secretion.Cells · 2021Article
- Clinical applications for exosomes: Are we there yet?British journal of pharmacology · 2021Review
- Revisiting Extracellular RNA Release, Processing, and Function.Trends in biochemical sciences · 2021Review
- Open Problems in Extracellular RNA Data Analysis: Insights From an ERCC Online Workshop.Frontiers in genetics · 2021Article
- Toward an Understanding of Extracellular tRNA Biology.Frontiers in molecular biosciences · 2021Article
- Foetal bovine serum influence on in vitro extracellular vesicle analyses.Journal of extracellular vesicles · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
In a recently published study, Anna Krichevsky and colleagues raise the important question of whether results of in vitro extracellular RNA (exRNA) studies, including extracellular vesicle (EV) investigations, are confounded by the presence of RNA in cell culture medium components such as foetal bovine serum (FBS). The answer, according to their data, is a resounding "yes". Even after lengthy ultracentrifugation to remove bovine EVs from FBS, the majority of exRNA in FBS remained. Although technical factors may affect the degree of depletion, residual EVs and exRNA in FBS could influence the conclusions of in vitro studies: certainly, for secreted RNA, and possibly also for cell-associated RNA. In this commentary, we critically examine some of the literature in this field, including a recent study from some of the authors of this piece, in light of the Wei et al. study and explore how cell culture-derived RNAs may affect what we think we know about EV RNAs. These findings hold particular consequence as the field moves towards a deeper understanding of EV-RNA associations and potential functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.