Evidence map›Paper›PMID 28326094›Full record

ArticleFrontiers in plant science2017

Selection and Validation of Reference Genes for Accurate RT-qPCR Data Normalization in

Madlles Q Martins, Ana S Fortunato, Weverton P Rodrigues, Fábio L Partelli, Eliemar Campostrini, Fernando C Lidon, Fábio M DaMatta, José C Ramalho, Ana I Ribeiro-Barros

Abstract read
In one paragraph

Article in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

  1. Article
  2. Genome-Wide Analysis of the GRF-GIF Module inInternational journal of molecular sciences · 2026
    Article
  3. Article
  4. Article
  5. Stress resilience inFrontiers in plant science · 2025
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Overexpression of Water-Responsive Genes Promoted by Elevated COInternational journal of molecular sciences · 2023
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Selection and validation of reference genes ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019
    Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Madlles Q MartinsPlant-Environment Interactions and Biodiversity Lab (PlantStress&Biodiversity), Linking Landscape, Environment, Agriculture and Food, Departmento de Recursos Naturais, Ambiente e Território, Instituto Superior de Agronomia, Universidade de Lisboa (ULisboa)Oeiras, Portugal; Programa de Pós-Graduação em Genética e Melhoramento, Centro de Ciências Agrárias e Engenharias, Universidade Federal do Espírito SantoAlegre, Brazil.
Ana S FortunatoPlant-Environment Interactions and Biodiversity Lab (PlantStress&Biodiversity), Linking Landscape, Environment, Agriculture and Food, Departmento de Recursos Naturais, Ambiente e Território, Instituto Superior de Agronomia, Universidade de Lisboa (ULisboa) Oeiras, Portugal.
Weverton P RodriguesPlant-Environment Interactions and Biodiversity Lab (PlantStress&Biodiversity), Linking Landscape, Environment, Agriculture and Food, Departmento de Recursos Naturais, Ambiente e Território, Instituto Superior de Agronomia, Universidade de Lisboa (ULisboa)Oeiras, Portugal; Setor Fisiologia Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual Norte Fluminense-Darcy RibeiroRio de Janeiro, Brazil.
Fábio L PartelliDepartmento de Ciências Agrárias e Biológicas, Centro Universitário Norte do Espírito Santo, Universidade Federal Espírito Santo São Mateus, Brazil.
Eliemar CampostriniSetor Fisiologia Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual Norte Fluminense-Darcy Ribeiro Rio de Janeiro, Brazil.
Fernando C LidonGeoBioTec, Departmento de Ciências da Terra, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa Monte da Caparica, Portugal.
Fábio M DaMattaDepartmento de Biologia Vegetal, Universidade Federal Viçosa Viçosa, Brazil.
José C RamalhoPlant-Environment Interactions and Biodiversity Lab (PlantStress&Biodiversity), Linking Landscape, Environment, Agriculture and Food, Departmento de Recursos Naturais, Ambiente e Território, Instituto Superior de Agronomia, Universidade de Lisboa (ULisboa)Oeiras, Portugal; GeoBioTec, Departmento de Ciências da Terra, Faculdade de Ciências e Tecnologia, Universidade NOVA de LisboaMonte da Caparica, Portugal.
Ana I Ribeiro-BarrosPlant-Environment Interactions and Biodiversity Lab (PlantStress&Biodiversity), Linking Landscape, Environment, Agriculture and Food, Departmento de Recursos Naturais, Ambiente e Território, Instituto Superior de Agronomia, Universidade de Lisboa (ULisboa)Oeiras, Portugal; GeoBioTec, Departmento de Ciências da Terra, Faculdade de Ciências e Tecnologia, Universidade NOVA de LisboaMonte da Caparica, Portugal.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

World coffee production has faced increasing challenges associated with ongoing climatic changes. Several studies, which have been almost exclusively based on temperature increase, have predicted extensive reductions (higher than half by 2,050) of actual coffee cropped areas. However, recent studies showed that elevated [CO

Indexed as

climate changescoffeeglobal warmingheat stressincreased air [CO2]normalization of transcriptomic studiesquantitative real-time PCRreference genes

Identifiers

PMID28326094
PMCPMC5339599

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.