ArticleScientific reports2017
Synergistic antibacterial mechanism of the Lactobacillus crispatus surface layer protein and nisin on Staphylococcus saprophyticus.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 35 citations in OpenAlex.
- Evaluation and mechanism of the antioxidant activity of lactic acid bacteria.Folia microbiologica · 2025Review
- A review on the ecological niche and functional properties of enterococci in health promotion.Current research in food science · 2025Review
- The Mechanism of Antimicrobial Activity of Conjugated Bile Acids against Lactic Acid Bacilli.Microorganisms · 2023Article
- Synergistic Effect of Combination of Various Microbial Hurdles in the Biopreservation of Meat and Meat Products-Systematic Review.Foods (Basel, Switzerland) · 2023Review
- Antibacterial and antibiofilm potential of Lacticaseibacillus rhamnosus YT and its cell-surface extract.BMC microbiology · 2023Article
- [Research Progress in the Relationship BetweenSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2022Review
- Synergistically-acting Enterocin LD3 and Plantaricin LD4 Against Gram-Positive and Gram-Negative Pathogenic Bacteria.Probiotics and antimicrobial proteins · 2021Article
- Composition of Vaginal Microbiota in Pregnant Women With Aerobic Vaginitis.Frontiers in cellular and infection microbiology · 2021Article
- S-layers: The Proteinaceous Multifunctional Armors of Gram-Positive Pathogens.Frontiers in microbiology · 2021Review
- Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components.Current microbiology · 2020Review
- Antibacterial activity and action mode of chlorogenic acid against Salmonella Enteritidis, a foodborne pathogen in chilled fresh chicken.World journal of microbiology & biotechnology · 2020Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SlpB, a surface layer protein isolated from Lactobacillus crispatus, has the potential to enhance the antimicrobial activity of nisin. Previous research indicated that, when combined with nisin, SlpB acted synergistically to inhibit Staphylococcus saprophyticus growth, thus extending the shelf life of chicken meat. In order to understand how SlpB enhances the antibacterial activity of nisin, electron microscopy, confocal laser scanning microscopy, flow cytometry and transmembrane electrical potential analysis were used to study cell wall organization and cell membrane integrity. No remarkable bacteriolytic effects were observed, indicating that cell death could not be attributed to cell lysis, although SlpB caused dramatic modifications of cell wall, thereby altering cell shape. The combination of SlpB and nisin also induced the release of ATP or UV-absorbing materials, as well as sudden dissipation of the transmembrane electrical potential by compromising membrane integrity. Considering that SlpB led to structural disorganization of the cell wall, and nisin access is enhanced to form a stable pore, cell death is a predictable outcome. SlpB significantly enhanced the effect of nisin at half of the minimum inhibitory concentration, which resulted in cell death by destroying the cell wall and cell membrane, therefore providing a new, feasible approach in food preservation.
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