Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells.

Adam J Trexler, Justin W Taraska

Open access · greenAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

4.9field-weighted citation impact, top 5% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

Structurally distinct endocytic pathways for B cell receptors in B lymphocytes.Molecular biology of the cell · 2020
Article
Local protein dynamics during microvesicle exocytosis in neuroendocrine cells.Molecular biology of the cell · 2018
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Adam J TrexlerLaboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, USA.

Justin W TaraskaLaboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50, Room 3312, 50 South Drive, Bethesda, MD, 20892, USA. justin.taraska@nih.gov.

National Heart Lung and Blood Institute · US

Funding

Architecture and control of vesicle fusion in excitable cellsZIAHL006098 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI TARASKA, JUSTIN · 2011 to 2025

$25.5M

Intramural NIH HHS ZIA HL006098

6 · The paper itself

Abstract

We describe a comprehensive method for imaging and analysis of local protein dynamics at single sites of exocytosis in living cultured endocrine cells. This method is well suited to quantitatively map the complex dynamics of individual molecules at single sites of vesicle fusion in live cells.

Indexed as

ExocytosisAnimalsBiological TransportCell LineEndocrine CellsGenes, ReporterHumansImage Processing, Computer-AssistedLuminescent ProteinsMicroscopy, FluorescenceLuminescent ProteinsEndocrine cellsExocytosisImage analysisLive-cell imagingTIRFVesicle fusion

Identifiers

PMID28324608

DOI10.1007/978-1-4939-6810-7_11

PMCPMC6298027

OpenAlexW2600683602

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.