Evidence map›Paper›PMID 28324522›Full record

Article3 Biotech2015

Detection of genetic variation in Ocimum species using RAPD and ISSR markers.

Hardik K Patel, Ranbir S Fougat, Sushil Kumar, Jigar G Mistry, Mukesh Kumar

Abstract read
In one paragraph

Article in 3 Biotech, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Article
  2. Article
  3. Geographical variation and genetic diversity ofFrontiers in plant science · 2024
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Assessment of genetic diversity inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019
    Article
  11. Article
  12. Article
  13. Article
  14. Evaluation of genetic diversity ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Hardik K PatelDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand, 388 110, India.
Ranbir S FougatDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand, 388 110, India. biotech.aau@gmail.com.
Sushil KumarDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand, 388 110, India.
Jigar G MistryDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand, 388 110, India.
Mukesh KumarDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand, 388 110, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There is a lack of information on the molecular characterization of Ocimum species and hence, efforts have been made under the present study to characterize 17 Ocimum genotypes belonging to 5 different species (O. basilicum, O. americanum, O. sanctum, O. gratissimum and O. Polystachyon) through random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. PCR amplification using 20 RAPD primers generated a total of 506 loci, of which 490 (96.47 %) loci were found polymorphic. The PIC value for RAPD ranged from 0.907 (OPF 14) to 0.954 (OPC 11) with an average of 0.937. The ISSR primers generated a total of 238 loci, of them 234 (98.17 %) loci were polymorphic. The PIC value ranged from 0.892 (UBC 808) to 0.943 (ISSR A12) with an average of 0.923. The average Jaccard's similarity coefficient based on RAPD and ISSR analysis was 0.58 and 0.52, respectively. Clustering pattern of dendrogram generated using the pooled RAPD and ISSR data showed all Ocimum genotypes in their respective species groups at a cutoff value of 0.49 and 0.42, respectively. Many unique species-specific alleles were amplified by RAPD and ISSR markers. In both marker systems, a maximum number of unique alleles were observed in O. sanctum. The results of the present investigation provided valid guidelines for collection, conservation and characterization of Ocimum genetic resources.

Indexed as

BasilDiversityISSROcimumRAPDTulsi

Identifiers

PMID28324522
PMCPMC4569613

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.