Article3 Biotech2015
Cloning, expression and characterization of L-asparaginase from Pseudomonas fluorescens for large scale production in E. coli BL21.
Article in 3 Biotech, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- A systematic review of recent trends in research on therapeutically significant L-asparaginase and acute lymphoblastic leukemia.Molecular biology reports · 2022Pooled it
- Novel glutaminase-free l-asparaginase fromRSC advances · 2026Article
- Recombinant L-asparaginase from Stenotrophomonas maltophilia: a promising low-immunogenic anticancer agent.Microbial cell factories · 2025Article
- Enzyme Engineering Strategies for the Bioenhancement of L-Asparaginase Used as a Biopharmaceutical.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2023Review
- Biochemical characterization of extremozyme L-asparaginase from Pseudomonas sp. PCH199 for therapeutics.AMB Express · 2023Article
- Characterization and applications of glutaminase free L-asparaginase from indigenous Bacillus halotolerans ASN9.PloS one · 2023Article
- Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems.Frontiers in pharmacology · 2023Review
- Appraisal of cytotoxicity and acrylamide mitigation potential of L-asparaginase SlpA from fish gut microbiome.Applied microbiology and biotechnology · 2022Article
- Scale-up fermentation of Escherichia coli for the production of recombinant endoglucanase from Clostridium thermocellum.Scientific reports · 2021Article
- Development of Processes for Recombinant L-Asparaginase II Production byPharmaceutics · 2020Article
- Article
- Recombinant l-Asparaginase II fromIndian journal of microbiology · 2019Article
- Validating the inhibitory effects of d- and l-serine on the enzyme activity of d-3-phosphoglycerate dehydrogenases that are purified fromGut pathogens · 2019Article
- In silico analysis, molecular cloning, expression and characterization of l-asparaginase gene from3 Biotech · 2017Article
- Current applications and different approaches for microbial l-asparaginase production.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2016Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-Asparaginase (E.C. 3.5.1.1) is used as an anti-neoplastic drug in the treatment of acute lymphoblastic leukemia. L-Asparaginase from Pseudomonas fluorescens was cloned and overexpressed in E. coli BL21. The Enzyme was found to be a Fusion protein-asparaginase complex which was given a lysozyme treatment and sonication, and then was purified in a Sepharose 6B column. The enzymatic properties of the recombinant enzyme were studied and the kinetic parameters were determined with kilometre of 109.99 mM and V
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