Article3 Biotech2015
Improved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera (L.) Dunal using sonication and heat treatment.
Article in 3 Biotech, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Development of Rhizobium rhizogenes-induced hairy root cultures of Vitex negundo for vitexin production.World journal of microbiology & biotechnology · 2026Article
- Reprogramming Hairy Root Cultures: A Synthetic Biology Framework for Precision Metabolite Biosynthesis.Plants (Basel, Switzerland) · 2025Review
- Optimization ofFrontiers in plant science · 2023Article
- Agrobacterium rhizogenes-mediated transformation of grain (Amaranthus hypochondriacus) and leafy (A. hybridus) amaranths.Plant cell reports · 2020Article
- Segregation ofFrontiers in plant science · 2020Article
- Improving the production of podophyllotoxin in hairy roots of Hyptis suaveolens induced from regenerated plantlets.PloS one · 2019Article
- Addressing Challenges to Enhance the Bioactives ofBioMed research international · 2017Review
- Review
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5 authors.
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Abstract
An improved Agrobacterium rhizogenes-mediated genetic transformation of Withania somnifera (L.) Dunal was developed using the bacterial strain R1000 with leaf segment explants of in vitro raised plantlets. Out of the three strains used (R1000, MTCC 2364 and MTCC 532), the strain R1000 proved to be more efficient than others. Among the different conditions tested, the highest (93.3 %) transformation rate was observed after 3 weeks when the explants were subjected to sonication (15 s) and heat treatment (41 °C for 5 min). Transgenic status of the hairy roots was confirmed by PCR using rol B-specific primers. HPLC analysis showed the ability of hairy roots to synthesize withaferin A and withanolide A, both steroidal lactones of medicinal value. This protocol offers new avenue in A. rhizogenes-mediated hairy root induction and is useful for large-scale production of these bioactive compounds from W. somnifera.
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